Development and evaluation of a real-time recombinase-aid amplification assay for rapid detection of Pseudomonas aeruginosa

Objective To establish a real-time recombinase-aid amplification (RAA) method for rapid detection of Pseudomonas aeruginosa. Methods Specific primers and exo probes based on ecfX gene of P. aeruginosa were designed in this study, and the validity of the method was evaluated by sensitivity, specifici...

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Bibliographic Details
Main Authors: Jinfeng WANG, Jialin XIANG, Xiaoxia SUN, Ruiwen LI, Yanfen JIANG, Zhimin CHEN, Libing LIU, Jianchang WANG, Wanzhe YUAN
Format: Article
Language:zho
Published: The Editorial Office of Chinese Journal of Food Hygiene 2020-09-01
Series:Zhongguo shipin weisheng zazhi
Subjects:
Online Access:http://www.zgspws.com/zgspwszzen/article/abstract/20200511