Whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniae
Abstract Microbial glycan microarrays (MGMs) populated with purified microbial glycans have been used to define the specificity of host immune factors toward microbes in a high throughput manner. However, a limitation of such arrays is that glycan presentation may not fully recapitulate the natural...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-27964-y |
| _version_ | 1827974659644063744 |
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| author | Shang-Chuen Wu Hau-Ming Jan Mary L. Vallecillo-Zúniga Matthew F. Rathgeber Caleb S. Stowell Kaleb L. Murdock Kashyap R. Patel Hirotomo Nakahara Carter J. Stowell Moon H. Nahm Connie M. Arthur Richard D. Cummings Sean R. Stowell |
| author_facet | Shang-Chuen Wu Hau-Ming Jan Mary L. Vallecillo-Zúniga Matthew F. Rathgeber Caleb S. Stowell Kaleb L. Murdock Kashyap R. Patel Hirotomo Nakahara Carter J. Stowell Moon H. Nahm Connie M. Arthur Richard D. Cummings Sean R. Stowell |
| author_sort | Shang-Chuen Wu |
| collection | DOAJ |
| description | Abstract Microbial glycan microarrays (MGMs) populated with purified microbial glycans have been used to define the specificity of host immune factors toward microbes in a high throughput manner. However, a limitation of such arrays is that glycan presentation may not fully recapitulate the natural presentation that exists on microbes. This raises the possibility that interactions observed on the array, while often helpful in predicting actual interactions with intact microbes, may not always accurately ascertain the overall affinity of a host immune factor for a given microbe. Using galectin-8 (Gal-8) as a probe, we compared the specificity and overall affinity observed using a MGM populated with glycans harvested from various strains of Streptococcus pneumoniae to an intact microbe microarray (MMA). Our results demonstrate that while similarities in binding specificity between the MGM and MMA are apparent, Gal-8 binding toward the MMA more accurately predicted interactions with strains of S. pneumoniae, including the overall specificity of Gal-8 antimicrobial activity. Taken together, these results not only demonstrate that Gal-8 possesses antimicrobial activity against distinct strains of S. pneumoniae that utilize molecular mimicry, but that microarray platforms populated with intact microbes present an advantageous strategy when exploring host interactions with microbes. |
| first_indexed | 2024-04-09T19:57:53Z |
| format | Article |
| id | doaj.art-c4280ad7b7c745f8a5a8f62ba788f3bd |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T19:57:53Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c4280ad7b7c745f8a5a8f62ba788f3bd2023-04-03T05:23:32ZengNature PortfolioScientific Reports2045-23222023-04-0113111410.1038/s41598-023-27964-yWhole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniaeShang-Chuen Wu0Hau-Ming Jan1Mary L. Vallecillo-Zúniga2Matthew F. Rathgeber3Caleb S. Stowell4Kaleb L. Murdock5Kashyap R. Patel6Hirotomo Nakahara7Carter J. Stowell8Moon H. Nahm9Connie M. Arthur10Richard D. Cummings11Sean R. Stowell12Joint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolDepartment of Medicine, University of Alabama at BirminghamJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolHarvard Glycomics Center, Beth Israel Deaconess Medical Center, Harvard Medical SchoolJoint Program in Transfusion Medicine, Department of Pathology, Brigham and Women’s Hospital, National Center for Functional Glycomics, 630E New Research Building, Harvard Medical SchoolAbstract Microbial glycan microarrays (MGMs) populated with purified microbial glycans have been used to define the specificity of host immune factors toward microbes in a high throughput manner. However, a limitation of such arrays is that glycan presentation may not fully recapitulate the natural presentation that exists on microbes. This raises the possibility that interactions observed on the array, while often helpful in predicting actual interactions with intact microbes, may not always accurately ascertain the overall affinity of a host immune factor for a given microbe. Using galectin-8 (Gal-8) as a probe, we compared the specificity and overall affinity observed using a MGM populated with glycans harvested from various strains of Streptococcus pneumoniae to an intact microbe microarray (MMA). Our results demonstrate that while similarities in binding specificity between the MGM and MMA are apparent, Gal-8 binding toward the MMA more accurately predicted interactions with strains of S. pneumoniae, including the overall specificity of Gal-8 antimicrobial activity. Taken together, these results not only demonstrate that Gal-8 possesses antimicrobial activity against distinct strains of S. pneumoniae that utilize molecular mimicry, but that microarray platforms populated with intact microbes present an advantageous strategy when exploring host interactions with microbes.https://doi.org/10.1038/s41598-023-27964-y |
| spellingShingle | Shang-Chuen Wu Hau-Ming Jan Mary L. Vallecillo-Zúniga Matthew F. Rathgeber Caleb S. Stowell Kaleb L. Murdock Kashyap R. Patel Hirotomo Nakahara Carter J. Stowell Moon H. Nahm Connie M. Arthur Richard D. Cummings Sean R. Stowell Whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniae Scientific Reports |
| title | Whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniae |
| title_full | Whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniae |
| title_fullStr | Whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniae |
| title_full_unstemmed | Whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniae |
| title_short | Whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin-8 toward distinct strains of Streptococcus pneumoniae |
| title_sort | whole microbe arrays accurately predict interactions and overall antimicrobial activity of galectin 8 toward distinct strains of streptococcus pneumoniae |
| url | https://doi.org/10.1038/s41598-023-27964-y |
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