Protocol to obtain high-quality single-cell RNA-sequencing data from mouse liver cells using centrifugation

Protocol to obtain high-quality single-cell RNA-sequencing data from mouse liver cells using centrifugation

Summary: High-quality single-cell RNA-sequencing data of liver cells, especially hepatocytes, are challenging due to cell death associated with hepatocyte isolation using fluorescence-activated cell sorting (FACS). Here, we present a protocol to obtain viable hepatocytes and nonparenchymal liver cel...

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Bibliographic Details
Main Author: Simeng Wang
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Genomics
RNAseq
Metabolism
Model Organisms
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007043

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http://www.sciencedirect.com/science/article/pii/S2666166722007043

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