Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease

Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease

CRISPR-Cas9 systems are being continually improved to enhance specificity and improve functionality. Here the authors design hybrid DNA-RNA guide and tracr molecules to direct Cas9 nuclease activity with reduced off-target effects.

Bibliographic Details
Main Authors: Fernando Orden Rueda, Michal Bista, Matthew D. Newton, Anne U. Goeppert, M. Emanuela Cuomo, Euan Gordon, Felix Kröner, Jon A. Read, Jonathan D. Wrigley, David Rueda, Benjamin J. M. Taylor
Format: Article
Language:English
Published: Nature Portfolio 2017-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-01732-9

Internet

https://doi.org/10.1038/s41467-017-01732-9

Similar Items