Construction of Aerobic/Anaerobic-Substrate-Induced Gene Expression Procedure for Exploration of Metagenomes From Subseafloor Sediments

Construction of Aerobic/Anaerobic-Substrate-Induced Gene Expression Procedure for Exploration of Metagenomes From Subseafloor Sediments

Substrate-induced gene expression (SIGEX) is a high-throughput promoter-trap method. It is a function-based metagenomic screening tool that relies on transcriptional activation of a reporter gene green fluorescence protein (gfp) by a metagenomic DNA library upon induction with a substrate. However,...

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Bibliographic Details
Main Authors: Taisuke Wakamatsu, Saki Mizobuchi, Fumiaki Mori, Taiki Futagami, Takeshi Terada, Yuki Morono
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Microbiology
Subjects:
substrate-induced gene expression
subseafloor
uncharacterized gene
anaerobic
halogenated compound
metagenome
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.726024/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2021.726024/full

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