Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems

Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems

Abstract Aspergillus niger, as an important industrial strain, is widely used in the production of a variety of organic acids and industrial enzymes. To excavate the greater potential of A. niger as a cell factory, the development of highly efficient genome editing techniques is crucial. Here, we de...

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Bibliographic Details
Main Authors: Yuan Zhang, Liming Ouyang, Yilin Nan, Ju Chu
Format: Article
Language:English
Published: SpringerOpen 2019-02-01
Series:Bioresources and Bioprocessing
Subjects:
Aspergillus niger
CRISPR/Cas9
Filamentous fungi
Genome editing
Ribozyme
pAN7-1
Online Access:http://link.springer.com/article/10.1186/s40643-019-0239-7

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http://link.springer.com/article/10.1186/s40643-019-0239-7

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