Semiautomated Clone Verification by Real-Time PCR Using Molecular Beacons
Conventional, high-throughput PCR analysis of common elements utilizing numerous primer sets and template DNA requires multiple rounds of PCR to ensure optimal conditions. Laborious gel electrophoresis and staining is then necessary to visualize amplification products. We propose novel multicolor mo...
| Autori principali: | , , , , , |
|---|---|
| Natura: | Articolo |
| Lingua: | English |
| Pubblicazione: |
Taylor & Francis Group
2000-12-01
|
| Serie: | BioTechniques |
| Accesso online: | https://www.future-science.com/doi/10.2144/00296rr01 |