| Summary: | Olive mill wastewater polyphenol extract was tested for antimicrobial activity against 64 strains of <em>Pseudomonas fluorescens</em> responsible for mozzarella discolouration. The extract showed a minimum inhibitory concentration (MIC)<sub>50</sub> value of 5 mg/mL and a MIC<sub>90</sub> value of 7 mg/mL. The MBC<sub>50</sub> and MBC<sub>90</sub> values corresponded to 6 and 8 mg/mL, respectively. The MIC concentration (7 mg/mL) was demonstrated to have a bacteriostatic effect while maintaining the bacterial concentration on the levels of the inoculum for 48 hours. The 3/2 MIC concentration was responsible for four logs CFU/mL depletion in colony count after 24 h. As the extract concentration decreased from MIC value, no inhibitory effects were recorded.
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