Visualization of Gene Reciprocity among Lactic Acid Bacteria in Yogurt by RNase H-Assisted Rolling Circle Amplification-Fluorescence <i>In Situ</i> Hybridization

Visualization of Gene Reciprocity among Lactic Acid Bacteria in Yogurt by RNase H-Assisted Rolling Circle Amplification-Fluorescence <i>In Situ</i> Hybridization

Recently, we developed an <i>in situ</i> mRNA detection method termed RNase H-assisted rolling circle amplification-fluorescence <i>in situ</i> hybridization (RHa-RCA-FISH), which can detect even short mRNA in a bacterial cell. However, because this FISH method is sensitive t...

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Bibliographic Details
Main Authors: Kyohei Horio, Hirokazu Takahashi, Toshiro Kobori, Kenshi Watanabe, Tsunehiro Aki, Yutaka Nakashimada, Yoshiko Okamura
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Microorganisms
Subjects:
mRNA
fluorescence <i>in situ</i> hybridization
lactic acid bacteria
single cell
symbioses
Online Access:https://www.mdpi.com/2076-2607/9/6/1208

Internet

https://www.mdpi.com/2076-2607/9/6/1208

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