Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice
Abstract Background ApoJ/clusterin is a multifunctional protein highly expressed in the brain. The implication of ApoJ in β-amyloid (Aβ) fibrillization and clearance in the context of Alzheimer’s disease has been widely studied, although the source and concentration of ApoJ that promotes or inhibits...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Alzheimer’s Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13195-019-0498-8 |
| _version_ | 1819113016216518656 |
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| author | Sofía Fernández de Retana Paula Marazuela Montse Solé Guillem Colell Anna Bonaterra Jose Luis Sánchez-Quesada Joan Montaner Daniel Maspoch Mary Cano-Sarabia Mar Hernández-Guillamon |
| author_facet | Sofía Fernández de Retana Paula Marazuela Montse Solé Guillem Colell Anna Bonaterra Jose Luis Sánchez-Quesada Joan Montaner Daniel Maspoch Mary Cano-Sarabia Mar Hernández-Guillamon |
| author_sort | Sofía Fernández de Retana |
| collection | DOAJ |
| description | Abstract Background ApoJ/clusterin is a multifunctional protein highly expressed in the brain. The implication of ApoJ in β-amyloid (Aβ) fibrillization and clearance in the context of Alzheimer’s disease has been widely studied, although the source and concentration of ApoJ that promotes or inhibits Aβ cerebral accumulation is not clear yet. ApoJ is abundant in plasma and approximately 20% can appear bound to HDL-particles. In this regard, the impact of plasmatic ApoJ and its lipidation status on cerebral β-amyloidosis is still not known. Hence, our main objective was to study the effect of a peripheral increase of free ApoJ or reconstituted HDL particles containing ApoJ in an experimental model of cerebral β-amyloidosis. Methods Fourteen-month-old APP23 transgenic mice were subjected to subchronic intravenous treatment with rHDL-rApoJ nanodiscs or free rApoJ for 1 month. Aβ concentration and distribution in the brain, as well as Aβ levels in plasma and CSF, were determined after treatments. Other features associated to AD pathology, such as neuronal loss and neuroinflammation, were also evaluated. Results Both ApoJ-based treatments prevented the Aβ accumulation in cerebral arteries and induced a decrease in total brain insoluble Aβ42 levels. The peripheral treatment with rApoJ also induced an increase in the Aβ40 levels in CSF, whereas the concentration remained unaltered in plasma. At all the endpoints studied, the lipidation of rApoJ did not enhance the protective properties of free rApoJ. The effects obtained after subchronic treatment with free rApoJ were accompanied by a reduction in hippocampal neuronal loss and an enhancement of the expression of a phagocytic marker in microglial cells surrounding Aβ deposits. Finally, despite the activation of this phagocytic phenotype, treatments did not induce a global neuroinflammatory status. In fact, free rApoJ treatment was able to reduce the levels of interleukin-17 (IL17) and keratinocyte chemoattractant (KC) chemokine in the brain. Conclusions Our results demonstrate that an increase in circulating human rApoJ induces a reduction of insoluble Aβ and CAA load in the brain of APP23 mice. Thus, our study suggests that peripheral interventions, based on treatments with multifunctional physiological chaperones, offer therapeutic opportunities to regulate the cerebral Aβ load. |
| first_indexed | 2024-12-22T04:22:42Z |
| format | Article |
| id | doaj.art-c567c14d5e924df8b03caf97cbc8ae71 |
| institution | Directory Open Access Journal |
| issn | 1758-9193 |
| language | English |
| last_indexed | 2024-12-22T04:22:42Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Alzheimer’s Research & Therapy |
| spelling | doaj.art-c567c14d5e924df8b03caf97cbc8ae712022-12-21T18:39:13ZengBMCAlzheimer’s Research & Therapy1758-91932019-05-0111111710.1186/s13195-019-0498-8Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 miceSofía Fernández de Retana0Paula Marazuela1Montse Solé2Guillem Colell3Anna Bonaterra4Jose Luis Sánchez-Quesada5Joan Montaner6Daniel Maspoch7Mary Cano-Sarabia8Mar Hernández-Guillamon9Neurovascular Research Laboratory, Vall d’Hebron Research Institute, Universitat Autònoma de BarcelonaNeurovascular Research Laboratory, Vall d’Hebron Research Institute, Universitat Autònoma de BarcelonaNeurovascular Research Laboratory, Vall d’Hebron Research Institute, Universitat Autònoma de BarcelonaNeurovascular Research Laboratory, Vall d’Hebron Research Institute, Universitat Autònoma de BarcelonaNeurovascular Research Laboratory, Vall d’Hebron Research Institute, Universitat Autònoma de BarcelonaCardiovascular Biochemistry Group, Research Institute of the Hospital de Sant Pau (IIB Sant Pau)Neurovascular Research Laboratory, Vall d’Hebron Research Institute, Universitat Autònoma de BarcelonaCatalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and the Barcelona Institute of Science and TechnologyCatalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and the Barcelona Institute of Science and TechnologyNeurovascular Research Laboratory, Vall d’Hebron Research Institute, Universitat Autònoma de BarcelonaAbstract Background ApoJ/clusterin is a multifunctional protein highly expressed in the brain. The implication of ApoJ in β-amyloid (Aβ) fibrillization and clearance in the context of Alzheimer’s disease has been widely studied, although the source and concentration of ApoJ that promotes or inhibits Aβ cerebral accumulation is not clear yet. ApoJ is abundant in plasma and approximately 20% can appear bound to HDL-particles. In this regard, the impact of plasmatic ApoJ and its lipidation status on cerebral β-amyloidosis is still not known. Hence, our main objective was to study the effect of a peripheral increase of free ApoJ or reconstituted HDL particles containing ApoJ in an experimental model of cerebral β-amyloidosis. Methods Fourteen-month-old APP23 transgenic mice were subjected to subchronic intravenous treatment with rHDL-rApoJ nanodiscs or free rApoJ for 1 month. Aβ concentration and distribution in the brain, as well as Aβ levels in plasma and CSF, were determined after treatments. Other features associated to AD pathology, such as neuronal loss and neuroinflammation, were also evaluated. Results Both ApoJ-based treatments prevented the Aβ accumulation in cerebral arteries and induced a decrease in total brain insoluble Aβ42 levels. The peripheral treatment with rApoJ also induced an increase in the Aβ40 levels in CSF, whereas the concentration remained unaltered in plasma. At all the endpoints studied, the lipidation of rApoJ did not enhance the protective properties of free rApoJ. The effects obtained after subchronic treatment with free rApoJ were accompanied by a reduction in hippocampal neuronal loss and an enhancement of the expression of a phagocytic marker in microglial cells surrounding Aβ deposits. Finally, despite the activation of this phagocytic phenotype, treatments did not induce a global neuroinflammatory status. In fact, free rApoJ treatment was able to reduce the levels of interleukin-17 (IL17) and keratinocyte chemoattractant (KC) chemokine in the brain. Conclusions Our results demonstrate that an increase in circulating human rApoJ induces a reduction of insoluble Aβ and CAA load in the brain of APP23 mice. Thus, our study suggests that peripheral interventions, based on treatments with multifunctional physiological chaperones, offer therapeutic opportunities to regulate the cerebral Aβ load.http://link.springer.com/article/10.1186/s13195-019-0498-8ClusterinApolipoprotein JApoJAPP23Reconstituted HDLAlzheimer’s disease |
| spellingShingle | Sofía Fernández de Retana Paula Marazuela Montse Solé Guillem Colell Anna Bonaterra Jose Luis Sánchez-Quesada Joan Montaner Daniel Maspoch Mary Cano-Sarabia Mar Hernández-Guillamon Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice Alzheimer’s Research & Therapy Clusterin Apolipoprotein J ApoJ APP23 Reconstituted HDL Alzheimer’s disease |
| title | Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice |
| title_full | Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice |
| title_fullStr | Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice |
| title_full_unstemmed | Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice |
| title_short | Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice |
| title_sort | peripheral administration of human recombinant apoj clusterin modulates brain beta amyloid levels in app23 mice |
| topic | Clusterin Apolipoprotein J ApoJ APP23 Reconstituted HDL Alzheimer’s disease |
| url | http://link.springer.com/article/10.1186/s13195-019-0498-8 |
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