PEM-seq comprehensively quantifies DNA repair outcomes during gene-editing and DSB repair

PEM-seq comprehensively quantifies DNA repair outcomes during gene-editing and DSB repair

Summary: The repair products of double-stranded DNA breaks (DSBs) are crucial for investigating the mechanism underlying DNA damage repair as well as evaluating the safety and efficiency of gene-editing; however, a comprehensively quantitative assay remains to be established. Here, we describe the s...

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Bibliographic Details
Main Authors: Yang Liu, Jianhang Yin, Tingting Gan, Mengzhu Liu, Changchang Xin, Weiwei Zhang, Jiazhi Hu
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Bioinformatics
CRISPR
High Throughput Screening
Molecular Biology
Sequence analysis
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007942

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http://www.sciencedirect.com/science/article/pii/S2666166721007942

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