Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids

Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-as...

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Bibliographic Details
Main Authors: Daniel C. Volke, Nicolas T. Wirth, Pablo Ivan Nikel
Format: Article
Language:English
Published: Bio-protocol LLC 2021-02-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=3917&type=0