Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-as...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2021-02-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=3917&type=0 |