Establishing a 3D culture system for early organogenesis of monkey embryos ex vivo and single-cell transcriptome analysis of cultured embryos

Establishing a 3D culture system for early organogenesis of monkey embryos ex vivo and single-cell transcriptome analysis of cultured embryos

Summary: Creating in vitro culture platforms for monkey embryos is crucial for understanding the initial 4 weeks of early primate embryogenesis. Here, we present a protocol to culture cynomolgus monkey embryos in vitro for 25 days post-fertilization and to delineate the key developmental events of g...

Full description

Bibliographic Details
Main Authors: Nianqin Sun, Honglian Shao, Youyue Zhang, Baiquan Ci, Hui Yao, Bing Bai, Tao Tan
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence analysis
Cell Biology
Cell culture
Cell isolation
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672300802X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672300802X

Similar Items