Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology
Quantitative bioanalysis is essential when establishing pharmacokinetic properties during the drug development process. To overcome challenges of sensitivity, specificity and process complexity associated with the conventional analysis of antisense oligonucleotides (ASOs), a new approach to nonenzym...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-07-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0005 |
| _version_ | 1827008142942142464 |
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| author | Masako Osawa Takurou Akiya Funa Ogawa Takao Suzuki Masaki Yamagami Tadashi Umemoto Akira Ideno |
| author_facet | Masako Osawa Takurou Akiya Funa Ogawa Takao Suzuki Masaki Yamagami Tadashi Umemoto Akira Ideno |
| author_sort | Masako Osawa |
| collection | DOAJ |
| description | Quantitative bioanalysis is essential when establishing pharmacokinetic properties during the drug development process. To overcome challenges of sensitivity, specificity and process complexity associated with the conventional analysis of antisense oligonucleotides (ASOs), a new approach to nonenzymatic hybridization assays using probe alteration-linked self-assembly reaction (PALSAR) technology as a signal amplifier was evaluated. PALSAR quantification of ASOs in mouse tissue and plasma was able to achieve a high sensitivity ranging from 1.5 to 6 pg/ml, intra-/interday accuracies in the range of 86.8–119.1% and 88.1–113.1%, respectively, and precision of ≤17.2%. Furthermore, crossreactivity of 3′n-1, a metabolite with a single base difference, was <1%. Our approach provides an auspicious method for distinguishing metabolites and detecting ASOs with high sensitivity and specificity. |
| first_indexed | 2024-03-12T14:34:37Z |
| format | Article |
| id | doaj.art-c59bd1e7c8ef452f9e11b7123f8f6485 |
| institution | Directory Open Access Journal |
| issn | 0736-6205 1940-9818 |
| language | English |
| last_indexed | 2025-02-18T12:28:41Z |
| publishDate | 2023-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj.art-c59bd1e7c8ef452f9e11b7123f8f64852024-11-02T04:16:36ZengTaylor & Francis GroupBioTechniques0736-62051940-98182023-07-01751213010.2144/btn-2023-0005Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technologyMasako Osawa0Takurou Akiya1Funa Ogawa2Takao Suzuki3Masaki Yamagami4Tadashi Umemoto5Akira Ideno61New Technology, Tsukuba Research Institute, Research & Development, Sekisui Medical Co., Ltd, Inashiki-gun, Ibaraki, 300-1155, Japan1New Technology, Tsukuba Research Institute, Research & Development, Sekisui Medical Co., Ltd, Inashiki-gun, Ibaraki, 300-1155, Japan1New Technology, Tsukuba Research Institute, Research & Development, Sekisui Medical Co., Ltd, Inashiki-gun, Ibaraki, 300-1155, Japan2Luxna Biotech Co., Ltd, Suita, Osaka, 565-0871, Japan2Luxna Biotech Co., Ltd, Suita, Osaka, 565-0871, Japan2Luxna Biotech Co., Ltd, Suita, Osaka, 565-0871, Japan1New Technology, Tsukuba Research Institute, Research & Development, Sekisui Medical Co., Ltd, Inashiki-gun, Ibaraki, 300-1155, JapanQuantitative bioanalysis is essential when establishing pharmacokinetic properties during the drug development process. To overcome challenges of sensitivity, specificity and process complexity associated with the conventional analysis of antisense oligonucleotides (ASOs), a new approach to nonenzymatic hybridization assays using probe alteration-linked self-assembly reaction (PALSAR) technology as a signal amplifier was evaluated. PALSAR quantification of ASOs in mouse tissue and plasma was able to achieve a high sensitivity ranging from 1.5 to 6 pg/ml, intra-/interday accuracies in the range of 86.8–119.1% and 88.1–113.1%, respectively, and precision of ≤17.2%. Furthermore, crossreactivity of 3′n-1, a metabolite with a single base difference, was <1%. Our approach provides an auspicious method for distinguishing metabolites and detecting ASOs with high sensitivity and specificity.https://www.future-science.com/doi/10.2144/btn-2023-0005antisense oligonucleotidebioanalysisligand-binding assaynonenzymatic hybridization assayPALSAR technologysensitivity |
| spellingShingle | Masako Osawa Takurou Akiya Funa Ogawa Takao Suzuki Masaki Yamagami Tadashi Umemoto Akira Ideno Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology BioTechniques antisense oligonucleotide bioanalysis ligand-binding assay nonenzymatic hybridization assay PALSAR technology sensitivity |
| title | Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology |
| title_full | Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology |
| title_fullStr | Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology |
| title_full_unstemmed | Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology |
| title_short | Sensitive and specific quantification of antisense oligonucleotides using probe alteration-linked self-assembly reaction technology |
| title_sort | sensitive and specific quantification of antisense oligonucleotides using probe alteration linked self assembly reaction technology |
| topic | antisense oligonucleotide bioanalysis ligand-binding assay nonenzymatic hybridization assay PALSAR technology sensitivity |
| url | https://www.future-science.com/doi/10.2144/btn-2023-0005 |
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