Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems
Abstract Certain strains of Escherichia coli possess and express the toxin colibactin (Clb) which induces host mutations identical to the signature mutations of colorectal cancer (CRC) that lead to tumorigenic lesions. Since cattle are a known reservoir of several Enterobacteriaceae including E. col...
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| Format: | Article |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09274-x |
| _version_ | 1818774178539503616 |
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| author | Manita Guragain John W. Schmidt Norasak Kalchayanand Aaron M. Dickey Joseph M. Bosilevac |
| author_facet | Manita Guragain John W. Schmidt Norasak Kalchayanand Aaron M. Dickey Joseph M. Bosilevac |
| author_sort | Manita Guragain |
| collection | DOAJ |
| description | Abstract Certain strains of Escherichia coli possess and express the toxin colibactin (Clb) which induces host mutations identical to the signature mutations of colorectal cancer (CRC) that lead to tumorigenic lesions. Since cattle are a known reservoir of several Enterobacteriaceae including E. coli, this study screened for clb amongst E. coli isolated from colons of cattle-at-harvest (entering beef processing facility; n = 1430), across the beef processing continuum (feedlot to finished subprimal beef; n = 232), and in ground beef (n = 1074). Results demonstrated that clb + E. coli were present in cattle and beef. Prevalence of clb + E. coli from colonic contents of cattle and ground beef was 18.3% and 5.5%, respectively. clb + E. coli were found susceptible to commonly used meat processing interventions. Whole genome sequencing of 54 bovine and beef clb + isolates showed clb occurred in diverse genetic backgrounds, most frequently in phylogroup B1 (70.4%), MLST 1079 (42.6%), and serogroup O49 (40.7%). |
| first_indexed | 2024-12-18T10:37:01Z |
| format | Article |
| id | doaj.art-c5a95e5977f4447db4ccfe7319bdeb59 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T10:37:01Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c5a95e5977f4447db4ccfe7319bdeb592022-12-21T21:10:44ZengNature PortfolioScientific Reports2045-23222022-03-0112111210.1038/s41598-022-09274-xCharacterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systemsManita Guragain0John W. Schmidt1Norasak Kalchayanand2Aaron M. Dickey3Joseph M. Bosilevac4Meat Safety and Quality Research Unit, US Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research CenterMeat Safety and Quality Research Unit, US Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research CenterMeat Safety and Quality Research Unit, US Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research CenterMeat Safety and Quality Research Unit, US Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research CenterMeat Safety and Quality Research Unit, US Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research CenterAbstract Certain strains of Escherichia coli possess and express the toxin colibactin (Clb) which induces host mutations identical to the signature mutations of colorectal cancer (CRC) that lead to tumorigenic lesions. Since cattle are a known reservoir of several Enterobacteriaceae including E. coli, this study screened for clb amongst E. coli isolated from colons of cattle-at-harvest (entering beef processing facility; n = 1430), across the beef processing continuum (feedlot to finished subprimal beef; n = 232), and in ground beef (n = 1074). Results demonstrated that clb + E. coli were present in cattle and beef. Prevalence of clb + E. coli from colonic contents of cattle and ground beef was 18.3% and 5.5%, respectively. clb + E. coli were found susceptible to commonly used meat processing interventions. Whole genome sequencing of 54 bovine and beef clb + isolates showed clb occurred in diverse genetic backgrounds, most frequently in phylogroup B1 (70.4%), MLST 1079 (42.6%), and serogroup O49 (40.7%).https://doi.org/10.1038/s41598-022-09274-x |
| spellingShingle | Manita Guragain John W. Schmidt Norasak Kalchayanand Aaron M. Dickey Joseph M. Bosilevac Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems Scientific Reports |
| title | Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems |
| title_full | Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems |
| title_fullStr | Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems |
| title_full_unstemmed | Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems |
| title_short | Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems |
| title_sort | characterization of escherichia coli harboring colibactin genes clb isolated from beef production and processing systems |
| url | https://doi.org/10.1038/s41598-022-09274-x |
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