Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution
Abstract We recently reported that strong activation of the optogenetic chloride pump, halorhodopsin leads to a secondary redistribution of K+ ions into the cell, through tonically open, “leak” K+ channels. Here we show that this effect is not unique to halorhodopsin but is also seen with activation...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-08-01
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| Series: | Physiological Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.14814/phy2.15778 |
| _version_ | 1827590166090350592 |
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| author | R. Ryley Parrish Tom Jackson‐Taylor Juha Voipio Andrew J. Trevelyan |
| author_facet | R. Ryley Parrish Tom Jackson‐Taylor Juha Voipio Andrew J. Trevelyan |
| author_sort | R. Ryley Parrish |
| collection | DOAJ |
| description | Abstract We recently reported that strong activation of the optogenetic chloride pump, halorhodopsin leads to a secondary redistribution of K+ ions into the cell, through tonically open, “leak” K+ channels. Here we show that this effect is not unique to halorhodopsin but is also seen with activation of another electrogenic ion pump, archaerhodopsin. The two opsins differ however in the size of the rebound rise in extracellular potassium, [K+]o, after the end of activation, which is far larger with halorhodopsin than for archaerhodopsin activation. Multiple linear regression modeling indicates that the variance in the postillumination surge in [K+]o was explained both by the size of the preceding, illumination‐induced drop in [K+]o and also by the type of opsin. These data provide additional support for the hypothesis that intense chloride‐loading of cells, as occurs naturally following intense bursts of GABAergic synaptic bombardment, or artificially following halorhodopsin activation, is followed by extrusion of both Cl− and K+ coupled together. We discuss this with respect to the pattern of [K+]o rise that occurs at the onset of seizure‐like events. |
| first_indexed | 2024-03-09T01:13:02Z |
| format | Article |
| id | doaj.art-c5afedcc324347b09c85827ee6daf979 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:13:02Z |
| publishDate | 2023-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-c5afedcc324347b09c85827ee6daf9792023-12-11T03:55:35ZengWileyPhysiological Reports2051-817X2023-08-011115n/an/a10.14814/phy2.15778Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistributionR. Ryley Parrish0Tom Jackson‐Taylor1Juha Voipio2Andrew J. Trevelyan3Medical School, Newcastle University Biosciences Institute Newcastle upon Tyne UKMedical School, Newcastle University Biosciences Institute Newcastle upon Tyne UKFaculty of Biological and Environmental Sciences, Molecular and Integrative Biosciences University of Helsinki Helsinki FinlandMedical School, Newcastle University Biosciences Institute Newcastle upon Tyne UKAbstract We recently reported that strong activation of the optogenetic chloride pump, halorhodopsin leads to a secondary redistribution of K+ ions into the cell, through tonically open, “leak” K+ channels. Here we show that this effect is not unique to halorhodopsin but is also seen with activation of another electrogenic ion pump, archaerhodopsin. The two opsins differ however in the size of the rebound rise in extracellular potassium, [K+]o, after the end of activation, which is far larger with halorhodopsin than for archaerhodopsin activation. Multiple linear regression modeling indicates that the variance in the postillumination surge in [K+]o was explained both by the size of the preceding, illumination‐induced drop in [K+]o and also by the type of opsin. These data provide additional support for the hypothesis that intense chloride‐loading of cells, as occurs naturally following intense bursts of GABAergic synaptic bombardment, or artificially following halorhodopsin activation, is followed by extrusion of both Cl− and K+ coupled together. We discuss this with respect to the pattern of [K+]o rise that occurs at the onset of seizure‐like events.https://doi.org/10.14814/phy2.15778archaerhodopsinchloridechloride‐cation‐cotransporterhalorhodopsinpotassium |
| spellingShingle | R. Ryley Parrish Tom Jackson‐Taylor Juha Voipio Andrew J. Trevelyan Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution Physiological Reports archaerhodopsin chloride chloride‐cation‐cotransporter halorhodopsin potassium |
| title | Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution |
| title_full | Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution |
| title_fullStr | Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution |
| title_full_unstemmed | Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution |
| title_short | Optogenetic ion pumps differ with respect to the secondary pattern of K+ redistribution |
| title_sort | optogenetic ion pumps differ with respect to the secondary pattern of k redistribution |
| topic | archaerhodopsin chloride chloride‐cation‐cotransporter halorhodopsin potassium |
| url | https://doi.org/10.14814/phy2.15778 |
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