Optimization of Real-Time PCR-melting for detection of the Cholesterol-deficiency mutation in Holstein Friesian cattle

The purpose of this study was to optimize a real-time PCR-melting analysis for reliable and economical detection of the 7.5 Kb mutant insert of the BoERVK bovine transposable element in exon 5 of the Apolipoprotein B (APOB) gene, which causes cholesterol deficiency — CD — (OMIA 001965-9913). This t...

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Bibliographic Details
Main Authors: Andrea Branda-Sica, Paula Nicolini, Rody Artigas, Maria Teresa Federici, Silvia Llambi
Format: Article
Language:English
Published: Universidad del Zulia 2022-06-01
Series:Revista Científica
Subjects:
Online Access:https://produccioncientificaluz.org/index.php/cientifica/article/view/38277