Construction of an Escherichia coli cell factory to synthesize taxadien-5α-ol, the key precursor of anti-cancer drug paclitaxel
Highlights We report a recombinant Escherichia coli BL21(DE3) strain for de novo production of taxadien-5α-ol, the key precursor of paclitaxel. Through screening of key enzymes and the fermentation condition optimization, the final production of total oxygenated taxanes was raised up to 27 mg L−1 in...
| Автори: | , , , , |
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| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
SpringerOpen
2022-08-01
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| Серія: | Bioresources and Bioprocessing |
| Предмети: | |
| Онлайн доступ: | https://doi.org/10.1186/s40643-022-00569-5 |
| Резюме: | Highlights We report a recombinant Escherichia coli BL21(DE3) strain for de novo production of taxadien-5α-ol, the key precursor of paclitaxel. Through screening of key enzymes and the fermentation condition optimization, the final production of total oxygenated taxanes was raised up to 27 mg L−1 in 50-mL flask cultivation, of which the yield of taxadien-5α-ol was 7.0 mg L−1, representing approximately a 12-fold and 23-fold improvements, respectively. It is believed that the strategy used in this study will guide in the synthesis of terpenoids. |
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| ISSN: | 2197-4365 |