MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway
Introduction In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs). Material and methods We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of m...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Termedia Publishing House
2022-11-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/MiRNA-21-promotes-differentiation-of-bone-marrow-mesenchymal-stem-cells-into-cardiomyocyte,154956,0,2.html |
| _version_ | 1797435342484144128 |
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| author | Wengang Yang Lei Zhao Song Xue Jidong Liu Jianggui Shan |
| author_facet | Wengang Yang Lei Zhao Song Xue Jidong Liu Jianggui Shan |
| author_sort | Wengang Yang |
| collection | DOAJ |
| description | Introduction
In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs).
Material and methods
We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of miRNA-21, ajuba, Isl1 and cTnI was detected by RT-qPCR, WB and immunofluorescence staining in groups.
Results
MiRNA-21 over-expression increased the expression of Isl1, and vice versa. Ajuba over-expression decreased the expression of Isl1, and vice versa. Ajuba negatively regulated the differentiation of BMSCs into cardiomyocyte-like cells.
Conclusions
MiRNA-21 could regulate differentiation of bone marrow mesenchymal stem cells (BMSCs) to cardiomyocyte-like cells through the ajuba/Isl1 axis pathway. |
| first_indexed | 2024-03-09T10:46:49Z |
| format | Article |
| id | doaj.art-c6157f506d094c399ca64f23c6df6d6f |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:46:49Z |
| publishDate | 2022-11-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-c6157f506d094c399ca64f23c6df6d6f2023-12-01T09:59:22ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512022-11-011861672167710.5114/aoms/154956154956MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathwayWengang Yang0Lei Zhao1Song Xue2Jidong Liu3Jianggui Shan4Department of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ChinaDepartment of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ChinaDepartment of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ChinaDepartment of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ChinaDepartment of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, ChinaIntroduction In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs). Material and methods We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of miRNA-21, ajuba, Isl1 and cTnI was detected by RT-qPCR, WB and immunofluorescence staining in groups. Results MiRNA-21 over-expression increased the expression of Isl1, and vice versa. Ajuba over-expression decreased the expression of Isl1, and vice versa. Ajuba negatively regulated the differentiation of BMSCs into cardiomyocyte-like cells. Conclusions MiRNA-21 could regulate differentiation of bone marrow mesenchymal stem cells (BMSCs) to cardiomyocyte-like cells through the ajuba/Isl1 axis pathway.https://www.archivesofmedicalscience.com/MiRNA-21-promotes-differentiation-of-bone-marrow-mesenchymal-stem-cells-into-cardiomyocyte,154956,0,2.htmlmirna-21ajuba/ isl1 axisdifferentiationbmscs |
| spellingShingle | Wengang Yang Lei Zhao Song Xue Jidong Liu Jianggui Shan MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway Archives of Medical Science mirna-21 ajuba/ isl1 axis differentiation bmscs |
| title | MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway |
| title_full | MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway |
| title_fullStr | MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway |
| title_full_unstemmed | MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway |
| title_short | MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway |
| title_sort | mirna 21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte like cells by regulating the ajuba isl1 axis pathway |
| topic | mirna-21 ajuba/ isl1 axis differentiation bmscs |
| url | https://www.archivesofmedicalscience.com/MiRNA-21-promotes-differentiation-of-bone-marrow-mesenchymal-stem-cells-into-cardiomyocyte,154956,0,2.html |
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