Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging.
Near-infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased scatterin...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-11-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3000965 |
| _version_ | 1818577592657117184 |
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| author | Yong Qian Danielle M Orozco Cosio Kiryl D Piatkevich Sarah Aufmkolk Wan-Chi Su Orhan T Celiker Anne Schohl Mitchell H Murdock Abhi Aggarwal Yu-Fen Chang Paul W Wiseman Edward S Ruthazer Edward S Boyden Robert E Campbell |
| author_facet | Yong Qian Danielle M Orozco Cosio Kiryl D Piatkevich Sarah Aufmkolk Wan-Chi Su Orhan T Celiker Anne Schohl Mitchell H Murdock Abhi Aggarwal Yu-Fen Chang Paul W Wiseman Edward S Ruthazer Edward S Boyden Robert E Campbell |
| author_sort | Yong Qian |
| collection | DOAJ |
| description | Near-infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report 2 improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and Caenorhabditis elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics. |
| first_indexed | 2024-12-16T06:32:22Z |
| format | Article |
| id | doaj.art-c61b9ef989474c1c9da003b377045026 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-16T06:32:22Z |
| publishDate | 2020-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-c61b9ef989474c1c9da003b3770450262022-12-21T22:40:52ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852020-11-011811e300096510.1371/journal.pbio.3000965Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging.Yong QianDanielle M Orozco CosioKiryl D PiatkevichSarah AufmkolkWan-Chi SuOrhan T CelikerAnne SchohlMitchell H MurdockAbhi AggarwalYu-Fen ChangPaul W WisemanEdward S RuthazerEdward S BoydenRobert E CampbellNear-infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report 2 improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and Caenorhabditis elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics.https://doi.org/10.1371/journal.pbio.3000965 |
| spellingShingle | Yong Qian Danielle M Orozco Cosio Kiryl D Piatkevich Sarah Aufmkolk Wan-Chi Su Orhan T Celiker Anne Schohl Mitchell H Murdock Abhi Aggarwal Yu-Fen Chang Paul W Wiseman Edward S Ruthazer Edward S Boyden Robert E Campbell Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. PLoS Biology |
| title | Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. |
| title_full | Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. |
| title_fullStr | Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. |
| title_full_unstemmed | Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. |
| title_short | Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. |
| title_sort | improved genetically encoded near infrared fluorescent calcium ion indicators for in vivo imaging |
| url | https://doi.org/10.1371/journal.pbio.3000965 |
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