Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animals
ABSTRACT: Milk-derived peptides have emerged as a popular mean to manage various lifestyle disorders such as diabetes. Fermentation is being explored as one of the faster and efficient way of producing peptides with antidiabetic potential. Therefore, in this study, an attempt was made to comparative...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030223012183 |
| _version_ | 1797201277090791424 |
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| author | Priti Mudgil Mouza Khamis Obaid Al Dhaheri Maitha Saif Mohammed Alsubousi Hina Khan Ali Ali Redha Pei-Gee Yap Chee-Yuen Gan Sajid Maqsood |
| author_facet | Priti Mudgil Mouza Khamis Obaid Al Dhaheri Maitha Saif Mohammed Alsubousi Hina Khan Ali Ali Redha Pei-Gee Yap Chee-Yuen Gan Sajid Maqsood |
| author_sort | Priti Mudgil |
| collection | DOAJ |
| description | ABSTRACT: Milk-derived peptides have emerged as a popular mean to manage various lifestyle disorders such as diabetes. Fermentation is being explored as one of the faster and efficient way of producing peptides with antidiabetic potential. Therefore, in this study, an attempt was made to comparatively investigate the pancreatic α-amylase (PAA) inhibitory properties of peptides derived from milk of different farm animals through probiotic fermentation. Peptide's identification was carried out using liquid chromatography-quadrupole time-of-flight mass spectrometry and inhibition mechanisms were characterized by molecular docking. Results obtained showed a PAA-IC50 value (the amount of protein equivalent needed to inhibit 50% of enzymes) between 2.39 and 36.1 µg protein equivalent for different fermented samples. Overall, Pediococcus pentosaceus MF000957-derived fermented milk from all animals indicated higher PAA inhibition than other probiotic derived fermented milk (PAA-IC50 values of 6.01, 3.53, 15.6, and 10.8 µg protein equivalent for bovine, camel, goat, and sheep fermented milk). Further, molecular docking analysis indicated that camel milk-derived peptide IMEQQQTEDEQQDK and goat milk-derived peptide DQHQKAMKPWTQPK were the most potent PAA inhibitory peptides. Overall, the study concluded that fermentation derived peptides may prove useful in for managing diabetes via inhibition of carbohydrate digesting enzyme PAA. |
| first_indexed | 2024-04-24T07:44:59Z |
| format | Article |
| id | doaj.art-c631e0f67e7048698c3100c8d7723624 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-04-24T07:44:59Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-c631e0f67e7048698c3100c8d77236242024-04-19T04:16:16ZengElsevierJournal of Dairy Science0022-03022024-05-01107526332652Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animalsPriti Mudgil0Mouza Khamis Obaid Al Dhaheri1Maitha Saif Mohammed Alsubousi2Hina Khan3Ali Ali Redha4Pei-Gee Yap5Chee-Yuen Gan6Sajid Maqsood7Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, 15551, United Arab Emirates; Corresponding authorsDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, 15551, United Arab EmiratesDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, 15551, United Arab EmiratesDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, 15551, United Arab EmiratesThe Department of Public Health and Sport Sciences, University of Exeter Medical School, Faculty of Health and Life Sciences, University of Exeter, Exeter, EX1 2LU, United Kingdom; Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Brisbane, QLD 4072, AustraliaAnalytical Biochemistry Research Centre (ABrC), Universiti Sains Malaysia, 11800, USM, Penang, MalaysiaAnalytical Biochemistry Research Centre (ABrC), Universiti Sains Malaysia, 11800, USM, Penang, MalaysiaDepartment of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, 15551, United Arab Emirates; Zayed Centre of Health Science, United Arab Emirates University, Al Ain 15551, United Arab Emirates; Corresponding authorsABSTRACT: Milk-derived peptides have emerged as a popular mean to manage various lifestyle disorders such as diabetes. Fermentation is being explored as one of the faster and efficient way of producing peptides with antidiabetic potential. Therefore, in this study, an attempt was made to comparatively investigate the pancreatic α-amylase (PAA) inhibitory properties of peptides derived from milk of different farm animals through probiotic fermentation. Peptide's identification was carried out using liquid chromatography-quadrupole time-of-flight mass spectrometry and inhibition mechanisms were characterized by molecular docking. Results obtained showed a PAA-IC50 value (the amount of protein equivalent needed to inhibit 50% of enzymes) between 2.39 and 36.1 µg protein equivalent for different fermented samples. Overall, Pediococcus pentosaceus MF000957-derived fermented milk from all animals indicated higher PAA inhibition than other probiotic derived fermented milk (PAA-IC50 values of 6.01, 3.53, 15.6, and 10.8 µg protein equivalent for bovine, camel, goat, and sheep fermented milk). Further, molecular docking analysis indicated that camel milk-derived peptide IMEQQQTEDEQQDK and goat milk-derived peptide DQHQKAMKPWTQPK were the most potent PAA inhibitory peptides. Overall, the study concluded that fermentation derived peptides may prove useful in for managing diabetes via inhibition of carbohydrate digesting enzyme PAA.http://www.sciencedirect.com/science/article/pii/S0022030223012183milk fermentationprobioticsα-amylase inhibitionantidiabetic bioactive peptidesmolecular docking |
| spellingShingle | Priti Mudgil Mouza Khamis Obaid Al Dhaheri Maitha Saif Mohammed Alsubousi Hina Khan Ali Ali Redha Pei-Gee Yap Chee-Yuen Gan Sajid Maqsood Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animals Journal of Dairy Science milk fermentation probiotics α-amylase inhibition antidiabetic bioactive peptides molecular docking |
| title | Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animals |
| title_full | Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animals |
| title_fullStr | Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animals |
| title_full_unstemmed | Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animals |
| title_short | Molecular docking studies on α-amylase inhibitory peptides from milk of different farm animals |
| title_sort | molecular docking studies on α amylase inhibitory peptides from milk of different farm animals |
| topic | milk fermentation probiotics α-amylase inhibition antidiabetic bioactive peptides molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S0022030223012183 |
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