Fermentation of Plant-Based Feeds with <i>Lactobacillus acidophilus</i> Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (<i>Oreochromis niloticus</i>) Reared in a Biofloc System
This study evaluated the effect of fermentation with <i>Lactobacillus acidophilus</i> on the biochemical and nutritional compositions of a plant-based diet and its effects on the productive performance and intestinal health of juvenile Nile tilapia (<i>Oreochromis niloticus</i&g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/2/332 |
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| author | Nataly Oliveira Dos Santos Neves Juliano De Dea Lindner Larissa Stockhausen Fernanda Regina Delziovo Mariana Bender Letícia Serzedello Luiz Augusto Cipriani Natalia Ha Everton Skoronski Enric Gisbert Ignasi Sanahuja Thiago El Hadi Perez Fabregat |
| author_facet | Nataly Oliveira Dos Santos Neves Juliano De Dea Lindner Larissa Stockhausen Fernanda Regina Delziovo Mariana Bender Letícia Serzedello Luiz Augusto Cipriani Natalia Ha Everton Skoronski Enric Gisbert Ignasi Sanahuja Thiago El Hadi Perez Fabregat |
| author_sort | Nataly Oliveira Dos Santos Neves |
| collection | DOAJ |
| description | This study evaluated the effect of fermentation with <i>Lactobacillus acidophilus</i> on the biochemical and nutritional compositions of a plant-based diet and its effects on the productive performance and intestinal health of juvenile Nile tilapia (<i>Oreochromis niloticus</i>) reared in a biofloc technology (BFT) system. The in vitro kinetics of feed fermentation were studied to determine the <i>L. acidophilus</i> growth and acidification curve through counting the colony-forming units (CFUs) mL<sup>−1</sup> and measuring the pH. Physicochemical and bromatological analyses of the feed were also performed. Based on the microbial growth kinetics results, vegetable-based Nile tilapia feeds fermented for 6 (FPB6) and 18 (FPB18) h were evaluated for 60 days. Fermented diets were compared with a positive control diet containing fishmeal (CFM) and a negative control diet without animal protein (CPB). Fermentation with <i>L. acidophilus</i> increased lactic acid bacteria (LAB) count and the soluble protein concentration of the plant-based feed, as well as decreasing the pH (<i>p</i> < 0.05). FPB treatments improved fish survival compared with CPB (<i>p</i> < 0.05). Fermentation increased feed intake but worsened feed efficiency (<i>p</i> < 0.05). The use of fermented feeds increased the LAB count and reduced pathogenic bacteria both in the BFT system’s water and in the animals’ intestines (<i>p</i> < 0.05). Fermented plant-based feeds showed greater villi (FPB6; FPB18) and higher goblet cell (FPB6) counts relative to the non-fermented plant-based feed, which may indicate improved intestinal health. The results obtained in this study are promising and show the sustainable potential of using fermented plant-based feeds in fish feeding rather than animal protein and, in particular, fishmeal. |
| first_indexed | 2024-03-08T11:07:44Z |
| format | Article |
| id | doaj.art-c63581c79a4e415fbf1d368ec7ab94d8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T11:07:44Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-c63581c79a4e415fbf1d368ec7ab94d82024-01-26T14:35:04ZengMDPI AGAnimals2076-26152024-01-0114233210.3390/ani14020332Fermentation of Plant-Based Feeds with <i>Lactobacillus acidophilus</i> Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (<i>Oreochromis niloticus</i>) Reared in a Biofloc SystemNataly Oliveira Dos Santos Neves0Juliano De Dea Lindner1Larissa Stockhausen2Fernanda Regina Delziovo3Mariana Bender4Letícia Serzedello5Luiz Augusto Cipriani6Natalia Ha7Everton Skoronski8Enric Gisbert9Ignasi Sanahuja10Thiago El Hadi Perez Fabregat11Department of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilDepartment of Food Science and Technology, Universidade Federal de Santa Catarina (UFSC), Rod. Admar Gonzaga, 1346, Bairro Itacorubi, Florianópolis 88034-000, SC, BrazilDepartment of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilDepartment of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilDepartment of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilDepartment of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilDepartment of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilDepartment of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilDepartment of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilAquaculture Program, Institute of Agrifood Research and Technology (IRTA-La Ràpita), Ctra. Poble Nou. Km 5.5, 43540 La Ràpita, SpainAquaculture Program, Institute of Agrifood Research and Technology (IRTA-La Ràpita), Ctra. Poble Nou. Km 5.5, 43540 La Ràpita, SpainDepartment of Animal Science (Pisciculture), Universidade do Estado de Santa Catarina (UDESC), Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, Lages 88520-000, SC, BrazilThis study evaluated the effect of fermentation with <i>Lactobacillus acidophilus</i> on the biochemical and nutritional compositions of a plant-based diet and its effects on the productive performance and intestinal health of juvenile Nile tilapia (<i>Oreochromis niloticus</i>) reared in a biofloc technology (BFT) system. The in vitro kinetics of feed fermentation were studied to determine the <i>L. acidophilus</i> growth and acidification curve through counting the colony-forming units (CFUs) mL<sup>−1</sup> and measuring the pH. Physicochemical and bromatological analyses of the feed were also performed. Based on the microbial growth kinetics results, vegetable-based Nile tilapia feeds fermented for 6 (FPB6) and 18 (FPB18) h were evaluated for 60 days. Fermented diets were compared with a positive control diet containing fishmeal (CFM) and a negative control diet without animal protein (CPB). Fermentation with <i>L. acidophilus</i> increased lactic acid bacteria (LAB) count and the soluble protein concentration of the plant-based feed, as well as decreasing the pH (<i>p</i> < 0.05). FPB treatments improved fish survival compared with CPB (<i>p</i> < 0.05). Fermentation increased feed intake but worsened feed efficiency (<i>p</i> < 0.05). The use of fermented feeds increased the LAB count and reduced pathogenic bacteria both in the BFT system’s water and in the animals’ intestines (<i>p</i> < 0.05). Fermented plant-based feeds showed greater villi (FPB6; FPB18) and higher goblet cell (FPB6) counts relative to the non-fermented plant-based feed, which may indicate improved intestinal health. The results obtained in this study are promising and show the sustainable potential of using fermented plant-based feeds in fish feeding rather than animal protein and, in particular, fishmeal.https://www.mdpi.com/2076-2615/14/2/332intestinal healthlactic acid bacteriasolid-state fermentationsoybean mealsurvival |
| spellingShingle | Nataly Oliveira Dos Santos Neves Juliano De Dea Lindner Larissa Stockhausen Fernanda Regina Delziovo Mariana Bender Letícia Serzedello Luiz Augusto Cipriani Natalia Ha Everton Skoronski Enric Gisbert Ignasi Sanahuja Thiago El Hadi Perez Fabregat Fermentation of Plant-Based Feeds with <i>Lactobacillus acidophilus</i> Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (<i>Oreochromis niloticus</i>) Reared in a Biofloc System Animals intestinal health lactic acid bacteria solid-state fermentation soybean meal survival |
| title | Fermentation of Plant-Based Feeds with <i>Lactobacillus acidophilus</i> Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (<i>Oreochromis niloticus</i>) Reared in a Biofloc System |
| title_full | Fermentation of Plant-Based Feeds with <i>Lactobacillus acidophilus</i> Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (<i>Oreochromis niloticus</i>) Reared in a Biofloc System |
| title_fullStr | Fermentation of Plant-Based Feeds with <i>Lactobacillus acidophilus</i> Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (<i>Oreochromis niloticus</i>) Reared in a Biofloc System |
| title_full_unstemmed | Fermentation of Plant-Based Feeds with <i>Lactobacillus acidophilus</i> Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (<i>Oreochromis niloticus</i>) Reared in a Biofloc System |
| title_short | Fermentation of Plant-Based Feeds with <i>Lactobacillus acidophilus</i> Improves the Survival and Intestinal Health of Juvenile Nile Tilapia (<i>Oreochromis niloticus</i>) Reared in a Biofloc System |
| title_sort | fermentation of plant based feeds with i lactobacillus acidophilus i improves the survival and intestinal health of juvenile nile tilapia i oreochromis niloticus i reared in a biofloc system |
| topic | intestinal health lactic acid bacteria solid-state fermentation soybean meal survival |
| url | https://www.mdpi.com/2076-2615/14/2/332 |
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