Development of a Curcumin-Loaded Lecithin/Chitosan Nanoparticle Utilizing a Box-Behnken Design of Experiment: Formulation Design and Influence of Process Parameters
Curcumin (CUR) has impressive pharmacologic properties, including cardioprotective, neuroprotective, antimicrobial, and anticancer activity. However, the pharmaceutical application of CUR is limited due to its poor aqueous solubility and low bioavailability. The development of novel formulations has...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/18/3758 |
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| author | Ismail A. Walbi Mohammad Zaki Ahmad Javed Ahmad Mohammed S. Algahtani Amer S. Alali Samar A. Alsudir Alhassan H. Aodah Hassan A. Albarqi |
| author_facet | Ismail A. Walbi Mohammad Zaki Ahmad Javed Ahmad Mohammed S. Algahtani Amer S. Alali Samar A. Alsudir Alhassan H. Aodah Hassan A. Albarqi |
| author_sort | Ismail A. Walbi |
| collection | DOAJ |
| description | Curcumin (CUR) has impressive pharmacologic properties, including cardioprotective, neuroprotective, antimicrobial, and anticancer activity. However, the pharmaceutical application of CUR is limited due to its poor aqueous solubility and low bioavailability. The development of novel formulations has attracted considerable attention to the idea of applying nanobiotechnology to improve the therapeutic efficacy of these challenging compounds. In this study, CUR-loaded lecithin–chitosan nanoparticles (CUR/LCSNPs) were developed and optimized by the concentration of chitosan, lecithin, and stirring speed by a 3-factorial Box-Behnken statistical design, resulting in an optimal concentration of chitosan (<i>A</i>) and lecithin (<i>B</i>) with a 1200 rpm stirring speed (<i>C</i>), with applied constraints of minimal average particle size (Y<sub>1</sub>), optimal zeta potential (Y<sub>2</sub>), and maximum entrapment efficiency (%EE) (Y<sub>3</sub>). The mean particle size of the checkpoint formulation ranged from 136.44 ± 1.74 nm to 267.94 ± 3.72, with a zeta potential of 18.5 ± 1.39 mV to 36.8 ± 3.24 mV and %EE of 69.84 ± 1.51% to 78.50 ± 2.11%. The mean particle size, zeta potential, %EE, and % cumulative drug release from the optimized formulation were 138.43 ± 2.09 nm, +18.98 ± 0.72 mV, 77.39 ± 1.70%, and 86.18 ± 1.5%, respectively. In vitro drug release followed the Korsmeyer–Peppas model with Fickian diffusion (<i>n</i> < 0.45). The optimized technique has proven successful, resulting in a nanoformulation that can be used for the high loading and controlled release of lipophilic drugs. |
| first_indexed | 2024-03-09T22:45:49Z |
| format | Article |
| id | doaj.art-c63b4e1c81b244dea6359019c1384af3 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T22:45:49Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-c63b4e1c81b244dea6359019c1384af32023-11-23T18:29:24ZengMDPI AGPolymers2073-43602022-09-011418375810.3390/polym14183758Development of a Curcumin-Loaded Lecithin/Chitosan Nanoparticle Utilizing a Box-Behnken Design of Experiment: Formulation Design and Influence of Process ParametersIsmail A. Walbi0Mohammad Zaki Ahmad1Javed Ahmad2Mohammed S. Algahtani3Amer S. Alali4Samar A. Alsudir5Alhassan H. Aodah6Hassan A. Albarqi7Department of Clinical Pharmacy, College of Pharmacy, Najran University, Najran 11001, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Najran University, Najran 11001, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Najran University, Najran 11001, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Najran University, Najran 11001, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kkharj 11942, Saudi ArabiaNational Center of Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaNational Center of Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Najran University, Najran 11001, Saudi ArabiaCurcumin (CUR) has impressive pharmacologic properties, including cardioprotective, neuroprotective, antimicrobial, and anticancer activity. However, the pharmaceutical application of CUR is limited due to its poor aqueous solubility and low bioavailability. The development of novel formulations has attracted considerable attention to the idea of applying nanobiotechnology to improve the therapeutic efficacy of these challenging compounds. In this study, CUR-loaded lecithin–chitosan nanoparticles (CUR/LCSNPs) were developed and optimized by the concentration of chitosan, lecithin, and stirring speed by a 3-factorial Box-Behnken statistical design, resulting in an optimal concentration of chitosan (<i>A</i>) and lecithin (<i>B</i>) with a 1200 rpm stirring speed (<i>C</i>), with applied constraints of minimal average particle size (Y<sub>1</sub>), optimal zeta potential (Y<sub>2</sub>), and maximum entrapment efficiency (%EE) (Y<sub>3</sub>). The mean particle size of the checkpoint formulation ranged from 136.44 ± 1.74 nm to 267.94 ± 3.72, with a zeta potential of 18.5 ± 1.39 mV to 36.8 ± 3.24 mV and %EE of 69.84 ± 1.51% to 78.50 ± 2.11%. The mean particle size, zeta potential, %EE, and % cumulative drug release from the optimized formulation were 138.43 ± 2.09 nm, +18.98 ± 0.72 mV, 77.39 ± 1.70%, and 86.18 ± 1.5%, respectively. In vitro drug release followed the Korsmeyer–Peppas model with Fickian diffusion (<i>n</i> < 0.45). The optimized technique has proven successful, resulting in a nanoformulation that can be used for the high loading and controlled release of lipophilic drugs.https://www.mdpi.com/2073-4360/14/18/3758curcuminchitosanlecithinnanoparticlesstirring speed3-factorial Box-Behnken statistical design |
| spellingShingle | Ismail A. Walbi Mohammad Zaki Ahmad Javed Ahmad Mohammed S. Algahtani Amer S. Alali Samar A. Alsudir Alhassan H. Aodah Hassan A. Albarqi Development of a Curcumin-Loaded Lecithin/Chitosan Nanoparticle Utilizing a Box-Behnken Design of Experiment: Formulation Design and Influence of Process Parameters Polymers curcumin chitosan lecithin nanoparticles stirring speed 3-factorial Box-Behnken statistical design |
| title | Development of a Curcumin-Loaded Lecithin/Chitosan Nanoparticle Utilizing a Box-Behnken Design of Experiment: Formulation Design and Influence of Process Parameters |
| title_full | Development of a Curcumin-Loaded Lecithin/Chitosan Nanoparticle Utilizing a Box-Behnken Design of Experiment: Formulation Design and Influence of Process Parameters |
| title_fullStr | Development of a Curcumin-Loaded Lecithin/Chitosan Nanoparticle Utilizing a Box-Behnken Design of Experiment: Formulation Design and Influence of Process Parameters |
| title_full_unstemmed | Development of a Curcumin-Loaded Lecithin/Chitosan Nanoparticle Utilizing a Box-Behnken Design of Experiment: Formulation Design and Influence of Process Parameters |
| title_short | Development of a Curcumin-Loaded Lecithin/Chitosan Nanoparticle Utilizing a Box-Behnken Design of Experiment: Formulation Design and Influence of Process Parameters |
| title_sort | development of a curcumin loaded lecithin chitosan nanoparticle utilizing a box behnken design of experiment formulation design and influence of process parameters |
| topic | curcumin chitosan lecithin nanoparticles stirring speed 3-factorial Box-Behnken statistical design |
| url | https://www.mdpi.com/2073-4360/14/18/3758 |
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