Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

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Summary: Understanding macrophage heterogeneity in tissue repair is a major challenge. Here, we describe a protocol that combines isolation of immune cells from skin wounds with subsequent flow-cytometry-based sorting of wound macrophages and single-cell RNA sequencing. We use a modified version of...

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Bibliographic Details
Main Authors: Sebastian Willenborg, Juliana G. Roscito, Alexander Gerbaulet, Axel Roers, Andreas Dahl, Sabine A. Eming, Susanne Reinhardt
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
Sequencing
RNAseq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002179

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http://www.sciencedirect.com/science/article/pii/S2666166722002179

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