Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hens
Toxic fungal species produce hazardous substances known as mycotoxins. Consumption of mycotoxin contaminated feed and food causes a variety of dangerous diseases and can even lead to death of animals and humans, raising global concerns for adverse health effects. To date, several strategies have bee...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254569/full |
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| author | Shahrbanou Hosseini Shahrbanou Hosseini Bertram Brenig Bertram Brenig Sunattinee Winitchakorn Chanidapha Kanmanee Orranee Srinual Orranee Srinual Wanaporn Tapingkae Wanaporn Tapingkae Kesinee Gatphayak Kesinee Gatphayak |
| author_facet | Shahrbanou Hosseini Shahrbanou Hosseini Bertram Brenig Bertram Brenig Sunattinee Winitchakorn Chanidapha Kanmanee Orranee Srinual Orranee Srinual Wanaporn Tapingkae Wanaporn Tapingkae Kesinee Gatphayak Kesinee Gatphayak |
| author_sort | Shahrbanou Hosseini |
| collection | DOAJ |
| description | Toxic fungal species produce hazardous substances known as mycotoxins. Consumption of mycotoxin contaminated feed and food causes a variety of dangerous diseases and can even lead to death of animals and humans, raising global concerns for adverse health effects. To date, several strategies have been developed to counteract with mycotoxin contamination. Red yeast as a novel biological dietary agent is a promising strategy to eliminate mycotoxicity in living organisms. Poultry are most susceptible animals to mycotoxin contamination, as they are fed a mixture of grains and are at higher risk of co-exposure to multiple toxic fungal substances. Therefore, this study investigated the genetic mechanism underlying long-term feeding with red yeast supplementation in interaction with multiple mycotoxins using transcriptome profiling (RNA_Seq) in the liver of laying hens. The results showed a high number of significantly differentially expressed genes in liver of chicken fed with a diet contaminated with mycotoxins, whereas the number of Significantly expressed genes was considerably reduced when the diet was supplemented with red yeast. The expression of genes involved in the phase I (CYP1A1, CYP1A2) and phase II (GSTA2, GSTA3, MGST1) detoxification process was downregulated in animals fed with mycotoxins contaminated diet, indicating suppression of the detoxification mechanisms. However, genes involved in antioxidant defense (GSTO1), apoptosis process (DUSP8), and tumor suppressor (KIAA1324, FBXO47, NME6) were upregulated in mycotoxins-exposed animals, suggesting activation of the antioxidant defense in response to mycotoxicity. Similarly, none of the detoxification genes were upregulated in hens fed with red yeast supplemented diet. However, neither genes involved in antioxidant defense nor tumor suppressor genes were expressed in the animals exposed to the red yeast supplemented feed, suggesting decreases the adsorption of biologically active mycotoxins in the liver of laying hens. We conclude that red yeast can act as a mycotoxin binder to decrease the adsorption of mycotoxins in the liver of laying hens and can be used as an effective strategy in the poultry feed industry to eliminate the adverse effects of mycotoxins for animals and increase food safety for human consumers. |
| first_indexed | 2024-03-12T02:09:34Z |
| format | Article |
| id | doaj.art-c66251bccab8416e9efaad51a48391e7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T02:09:34Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c66251bccab8416e9efaad51a48391e72023-09-06T17:43:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12545691254569Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hensShahrbanou Hosseini0Shahrbanou Hosseini1Bertram Brenig2Bertram Brenig3Sunattinee Winitchakorn4Chanidapha Kanmanee5Orranee Srinual6Orranee Srinual7Wanaporn Tapingkae8Wanaporn Tapingkae9Kesinee Gatphayak10Kesinee Gatphayak11Molecular Biology of Livestock and Molecular Diagnostics, Department of Animal Sciences, University of Goettingen, Göttingen, GermanyInstitute of Veterinary Medicine, University of Goettingen, Göttingen, GermanyMolecular Biology of Livestock and Molecular Diagnostics, Department of Animal Sciences, University of Goettingen, Göttingen, GermanyInstitute of Veterinary Medicine, University of Goettingen, Göttingen, GermanyDepartment of Animal and Aquatic Sciences, Chiang Mai University, Chiang Mai, ThailandDepartment of Animal and Aquatic Sciences, Chiang Mai University, Chiang Mai, ThailandDepartment of Animal and Aquatic Sciences, Chiang Mai University, Chiang Mai, ThailandFunctional Feed Innovation Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, ThailandDepartment of Animal and Aquatic Sciences, Chiang Mai University, Chiang Mai, ThailandFunctional Feed Innovation Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, ThailandDepartment of Animal and Aquatic Sciences, Chiang Mai University, Chiang Mai, ThailandFunctional Feed Innovation Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, ThailandToxic fungal species produce hazardous substances known as mycotoxins. Consumption of mycotoxin contaminated feed and food causes a variety of dangerous diseases and can even lead to death of animals and humans, raising global concerns for adverse health effects. To date, several strategies have been developed to counteract with mycotoxin contamination. Red yeast as a novel biological dietary agent is a promising strategy to eliminate mycotoxicity in living organisms. Poultry are most susceptible animals to mycotoxin contamination, as they are fed a mixture of grains and are at higher risk of co-exposure to multiple toxic fungal substances. Therefore, this study investigated the genetic mechanism underlying long-term feeding with red yeast supplementation in interaction with multiple mycotoxins using transcriptome profiling (RNA_Seq) in the liver of laying hens. The results showed a high number of significantly differentially expressed genes in liver of chicken fed with a diet contaminated with mycotoxins, whereas the number of Significantly expressed genes was considerably reduced when the diet was supplemented with red yeast. The expression of genes involved in the phase I (CYP1A1, CYP1A2) and phase II (GSTA2, GSTA3, MGST1) detoxification process was downregulated in animals fed with mycotoxins contaminated diet, indicating suppression of the detoxification mechanisms. However, genes involved in antioxidant defense (GSTO1), apoptosis process (DUSP8), and tumor suppressor (KIAA1324, FBXO47, NME6) were upregulated in mycotoxins-exposed animals, suggesting activation of the antioxidant defense in response to mycotoxicity. Similarly, none of the detoxification genes were upregulated in hens fed with red yeast supplemented diet. However, neither genes involved in antioxidant defense nor tumor suppressor genes were expressed in the animals exposed to the red yeast supplemented feed, suggesting decreases the adsorption of biologically active mycotoxins in the liver of laying hens. We conclude that red yeast can act as a mycotoxin binder to decrease the adsorption of mycotoxins in the liver of laying hens and can be used as an effective strategy in the poultry feed industry to eliminate the adverse effects of mycotoxins for animals and increase food safety for human consumers.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254569/fulldetoxificationfeed additivegene expressionlaying hensmycotoxinred yeast |
| spellingShingle | Shahrbanou Hosseini Shahrbanou Hosseini Bertram Brenig Bertram Brenig Sunattinee Winitchakorn Chanidapha Kanmanee Orranee Srinual Orranee Srinual Wanaporn Tapingkae Wanaporn Tapingkae Kesinee Gatphayak Kesinee Gatphayak Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hens Frontiers in Microbiology detoxification feed additive gene expression laying hens mycotoxin red yeast |
| title | Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hens |
| title_full | Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hens |
| title_fullStr | Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hens |
| title_full_unstemmed | Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hens |
| title_short | Genetic assessment of the effect of red yeast (Sporidiobolus pararoseus) as a feed additive on mycotoxin toxicity in laying hens |
| title_sort | genetic assessment of the effect of red yeast sporidiobolus pararoseus as a feed additive on mycotoxin toxicity in laying hens |
| topic | detoxification feed additive gene expression laying hens mycotoxin red yeast |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1254569/full |
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