Apical targeting of the formin Diaphanous in Drosophila tubular epithelia
Apical secretion from epithelial tubes of the Drosophila embryo is mediated by apical F-actin cables generated by the formin-family protein Diaphanous (Dia). Apical localization and activity of Dia are at the core of restricting F-actin formation to the correct membrane domain. Here we identify the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2013-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/00666 |
| _version_ | 1811200126524325888 |
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| author | Tal Rousso Annette M Shewan Keith E Mostov Eyal D Schejter Ben-Zion Shilo |
| author_facet | Tal Rousso Annette M Shewan Keith E Mostov Eyal D Schejter Ben-Zion Shilo |
| author_sort | Tal Rousso |
| collection | DOAJ |
| description | Apical secretion from epithelial tubes of the Drosophila embryo is mediated by apical F-actin cables generated by the formin-family protein Diaphanous (Dia). Apical localization and activity of Dia are at the core of restricting F-actin formation to the correct membrane domain. Here we identify the mechanisms that target Dia to the apical surface. PI(4,5)P2 levels at the apical membrane regulate Dia localization in both the MDCK cyst model and in Drosophila tubular epithelia. An N-terminal basic domain of Dia is crucial for apical localization, implying direct binding to PI(4,5)P2. Dia apical targeting also depends on binding to Rho1, which is critical for activation-induced conformational change, as well as physically anchoring Dia to the apical membrane. We demonstrate that binding to Rho1 facilitates interaction with PI(4,5)P2 at the plane of the membrane. Together these cues ensure efficient and distinct restriction of Dia to the apical membrane. |
| first_indexed | 2024-04-12T01:59:36Z |
| format | Article |
| id | doaj.art-c66273c97d0a4fdbab3fdc17b75b88e6 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:59:36Z |
| publishDate | 2013-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-c66273c97d0a4fdbab3fdc17b75b88e62022-12-22T03:52:43ZengeLife Sciences Publications LtdeLife2050-084X2013-07-01210.7554/eLife.00666Apical targeting of the formin Diaphanous in Drosophila tubular epitheliaTal Rousso0Annette M Shewan1Keith E Mostov2Eyal D Schejter3Ben-Zion Shilo4Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, IsraelDepartment of Anatomy, University of California, San Francisco, San Francisco, United StatesDepartment of Anatomy, University of California, San Francisco, San Francisco, United StatesDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot, IsraelApical secretion from epithelial tubes of the Drosophila embryo is mediated by apical F-actin cables generated by the formin-family protein Diaphanous (Dia). Apical localization and activity of Dia are at the core of restricting F-actin formation to the correct membrane domain. Here we identify the mechanisms that target Dia to the apical surface. PI(4,5)P2 levels at the apical membrane regulate Dia localization in both the MDCK cyst model and in Drosophila tubular epithelia. An N-terminal basic domain of Dia is crucial for apical localization, implying direct binding to PI(4,5)P2. Dia apical targeting also depends on binding to Rho1, which is critical for activation-induced conformational change, as well as physically anchoring Dia to the apical membrane. We demonstrate that binding to Rho1 facilitates interaction with PI(4,5)P2 at the plane of the membrane. Together these cues ensure efficient and distinct restriction of Dia to the apical membrane.https://elifesciences.org/articles/00666Diaphanousformintubular epitheliaapical localizationPI(4,5)P2Rho1 |
| spellingShingle | Tal Rousso Annette M Shewan Keith E Mostov Eyal D Schejter Ben-Zion Shilo Apical targeting of the formin Diaphanous in Drosophila tubular epithelia eLife Diaphanous formin tubular epithelia apical localization PI(4,5)P2 Rho1 |
| title | Apical targeting of the formin Diaphanous in Drosophila tubular epithelia |
| title_full | Apical targeting of the formin Diaphanous in Drosophila tubular epithelia |
| title_fullStr | Apical targeting of the formin Diaphanous in Drosophila tubular epithelia |
| title_full_unstemmed | Apical targeting of the formin Diaphanous in Drosophila tubular epithelia |
| title_short | Apical targeting of the formin Diaphanous in Drosophila tubular epithelia |
| title_sort | apical targeting of the formin diaphanous in drosophila tubular epithelia |
| topic | Diaphanous formin tubular epithelia apical localization PI(4,5)P2 Rho1 |
| url | https://elifesciences.org/articles/00666 |
| work_keys_str_mv | AT talrousso apicaltargetingoftheformindiaphanousindrosophilatubularepithelia AT annettemshewan apicaltargetingoftheformindiaphanousindrosophilatubularepithelia AT keithemostov apicaltargetingoftheformindiaphanousindrosophilatubularepithelia AT eyaldschejter apicaltargetingoftheformindiaphanousindrosophilatubularepithelia AT benzionshilo apicaltargetingoftheformindiaphanousindrosophilatubularepithelia |