Retro-orbital CD45 antibody labeling to evaluate antibody-secreting cell trafficking in mice

Summary: Antibody-secreting cells (ASCs) are critical regulators of the humoral immune response. However, differences between tissue resident populations versus those that have recently migrated to their final anatomic destination are poorly understood. Here, we present a protocol for using retro-or...

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Bibliographic Details
Main Authors: KimAnh T. Pioli, Peter D. Pioli
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002757