CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli

CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli

Abstract Background Synthetic biology heavily depends on rapid and simple techniques for DNA engineering, such as Ligase Cycling Reaction (LCR), Gibson assembly and Golden Gate assembly, all of which allow for fast, multi-fragment DNA assembly. A major enhancement of Golden Gate assembly is represen...

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Bibliographic Details
Main Authors: Stefano Vecchione, Georg Fritz
Format: Article
Language:English
Published: BMC 2019-11-01
Series:Journal of Biological Engineering
Subjects:
Synthetic biology
Chromosomal integration
CRIM plasmids
MoClo
CRIMoClo
Online Access:https://doi.org/10.1186/s13036-019-0218-8

Internet

https://doi.org/10.1186/s13036-019-0218-8

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