The His-tag as a decoy modulating preferred orientation in cryoEM

The His-tag as a decoy modulating preferred orientation in cryoEM

The His-tag is a widely used affinity tag that facilitates purification by means of affinity chromatography of recombinant proteins for functional and structural studies. We show here that His-tag presence affects how coproheme decarboxylase interacts with the air-water interface during grid prepara...

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Bibliographic Details
Main Authors: Raquel Bromberg, Kai Cai, Yirui Guo, Daniel Plymire, Tabitha Emde, Maciej Puzio, Dominika Borek, Zbyszek Otwinowski
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-10-01
Series:Frontiers in Molecular Biosciences
Subjects:
cryo-electron microscopy single particle reconstruction
cryoEM SPR
air-water interface
AWI
His-tag
preferred orientation
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2022.912072/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2022.912072/full

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