OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias.

Over the decades, practical biotechnology researchers have aimed to improve naturally occurring proteins and create novel ones. It is widely recognized that coupling protein sequence randomization with various effect screening methodologies is one of the most powerful techniques for quickly, efficie...

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Bibliographic Details
Main Authors: Artis Linars, Ivars Silamikelis, Dita Gudra, Ance Roga, Davids Fridmanis
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0262968