Testing protocols to optimize DNA extraction from tough leaf tissue: A case study in Encephalartos
Abstract Premise Plants with stiff, leathery leaves pose a challenge for standard DNA extraction protocols. These tissues are recalcitrant to mechanical disruption via TissueLyser (or analogous devices) and are often high in secondary metabolites. These compounding factors result in low yields, whic...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Wiley
2023-05-01
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Series: | Applications in Plant Sciences |
Subjects: | |
Online Access: | https://doi.org/10.1002/aps3.11525 |