Low-host double MDA workflow for uncultured ASFV positive blood and serum sample sequencing

African swine fever (ASF) is a highly lethal and contagious disease caused by African swine fever virus (ASFV). Whole-genome sequencing of ASFV is necessary to study its mutation, recombination, and trace its transmission. Uncultured samples have a considerable amount of background DNA, which causes...

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Bibliographic Details
Main Authors: Chengjun Zhang, Tangyu Cheng, Dongfan Li, Xuexiang Yu, Fangzhou Chen, Qigai He
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-09-01
Series:Frontiers in Veterinary Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2022.936781/full