Selection and Characterization of DNA Aptamers for Egg White Lysozyme

Selection and Characterization of DNA Aptamers for Egg White Lysozyme

We have selected aptamers binding to lysozyme from a DNA library using capillary electrophoresis-systematic evolution of ligands by exponential enrichment. During the selection process the dissociation constant of the ssDNA pool decreased from the micromolar to the low nanomolar range within five ro...

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Bibliographic Details
Main Authors: Jeroen Lammertyn, Ann Van Schepdael, Jozef Anné, Elke Lammertyn, Jeroen Pollet, Kris P. F. Janssen, Dinh T. Tran
Format: Article
Language:English
Published: MDPI AG 2010-03-01
Series:Molecules
Subjects:
aptamer
capillary electrophoresis
food allergen
lysozyme
systematic evolution of ligands by exponential enrichment
Online Access:http://www.mdpi.com/1420-3049/15/3/1127/

Internet

http://www.mdpi.com/1420-3049/15/3/1127/

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