Investigation of membrane protein—protein interactions using correlative FRET-PLA

Fluorescence resonance energy transfer (FRET) analysis and the recently developed proximity ligation assay (PLA) are widely used to study protein—protein interactions in situ. We have developed correlative FRET-PLA to monitor interactions between membrane proteins that frequently cause problems in c...

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Bibliographic Details
Main Authors: Daniel Ivanusic, Magdalena Eschricht, Joachim Denner
Format: Article
Language:English
Published: Taylor & Francis Group 2014-10-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000114215