Comprehensive metabolome analysis of intracellular metabolites in cultured cells

Comprehensive metabolome analysis of intracellular metabolites in cultured cells

Summary: Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation...

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Bibliographic Details
Main Authors: Ryuhei Kudo, Kaori Igarashi, Tomoyoshi Soga, Takamasa Ishikawa, Yasuhiro Saito
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Metabolism
Metabolomics
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004117

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http://www.sciencedirect.com/science/article/pii/S2666166722004117

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