Comprehensive metabolome analysis of intracellular metabolites in cultured cells
Summary: Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722004117 |
| _version_ | 1811225854684954624 |
|---|---|
| author | Ryuhei Kudo Kaori Igarashi Tomoyoshi Soga Takamasa Ishikawa Yasuhiro Saito |
| author_facet | Ryuhei Kudo Kaori Igarashi Tomoyoshi Soga Takamasa Ishikawa Yasuhiro Saito |
| author_sort | Ryuhei Kudo |
| collection | DOAJ |
| description | Summary: Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation for LC-MS and CE-MS. Here, we present a protocol to prepare for sequentially analyzing the metabolites from one sample. Here we describe the steps for breast cancer cell lines, MCF-7 cells, but the protocol can be applied to other cell types. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-12T09:14:13Z |
| format | Article |
| id | doaj.art-c6fb5a7ce424436cbf059c205c84e541 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-12T09:14:13Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c6fb5a7ce424436cbf059c205c84e5412022-12-22T03:38:53ZengElsevierSTAR Protocols2666-16672022-09-0133101531Comprehensive metabolome analysis of intracellular metabolites in cultured cellsRyuhei Kudo0Kaori Igarashi1Tomoyoshi Soga2Takamasa Ishikawa3Yasuhiro Saito4Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan; Infinity Lab Co., Ltd., Tsuruoka, Yamagata 997-0052, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan; Corresponding authorInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan; Infinity Lab Co., Ltd., Tsuruoka, Yamagata 997-0052, Japan; Corresponding authorInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan; Corresponding authorSummary: Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation for LC-MS and CE-MS. Here, we present a protocol to prepare for sequentially analyzing the metabolites from one sample. Here we describe the steps for breast cancer cell lines, MCF-7 cells, but the protocol can be applied to other cell types. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722004117Cell BiologyCell cultureMetabolismMetabolomicsMass Spectrometry |
| spellingShingle | Ryuhei Kudo Kaori Igarashi Tomoyoshi Soga Takamasa Ishikawa Yasuhiro Saito Comprehensive metabolome analysis of intracellular metabolites in cultured cells STAR Protocols Cell Biology Cell culture Metabolism Metabolomics Mass Spectrometry |
| title | Comprehensive metabolome analysis of intracellular metabolites in cultured cells |
| title_full | Comprehensive metabolome analysis of intracellular metabolites in cultured cells |
| title_fullStr | Comprehensive metabolome analysis of intracellular metabolites in cultured cells |
| title_full_unstemmed | Comprehensive metabolome analysis of intracellular metabolites in cultured cells |
| title_short | Comprehensive metabolome analysis of intracellular metabolites in cultured cells |
| title_sort | comprehensive metabolome analysis of intracellular metabolites in cultured cells |
| topic | Cell Biology Cell culture Metabolism Metabolomics Mass Spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722004117 |
| work_keys_str_mv | AT ryuheikudo comprehensivemetabolomeanalysisofintracellularmetabolitesinculturedcells AT kaoriigarashi comprehensivemetabolomeanalysisofintracellularmetabolitesinculturedcells AT tomoyoshisoga comprehensivemetabolomeanalysisofintracellularmetabolitesinculturedcells AT takamasaishikawa comprehensivemetabolomeanalysisofintracellularmetabolitesinculturedcells AT yasuhirosaito comprehensivemetabolomeanalysisofintracellularmetabolitesinculturedcells |