Comprehensive metabolome analysis of intracellular metabolites in cultured cells
Summary: Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation...
Main Authors: | Ryuhei Kudo, Kaori Igarashi, Tomoyoshi Soga, Takamasa Ishikawa, Yasuhiro Saito |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-09-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722004117 |
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