A Possible Inhibitory Role of Sialic Acid on MUC1 in Peritoneal Dissemination of Clear Cell-Type Ovarian Cancer Cells
The role of sialic acids on MUC1 in peritoneal dissemination of ovarian cancer cells was investigated. A human ovarian carcinoma cell line, ES-2, was transfected with full-length MUC1 containing 22 or 42 tandem repeats. These transfectants were less adherent to monolayers of patient-derived mesothel...
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/19/5962 |
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| author | Yutaka Tamada Hiroyuki Nomura Daisuke Aoki Tatsuro Irimura |
| author_facet | Yutaka Tamada Hiroyuki Nomura Daisuke Aoki Tatsuro Irimura |
| author_sort | Yutaka Tamada |
| collection | DOAJ |
| description | The role of sialic acids on MUC1 in peritoneal dissemination of ovarian cancer cells was investigated. A human ovarian carcinoma cell line, ES-2, was transfected with full-length MUC1 containing 22 or 42 tandem repeats. These transfectants were less adherent to monolayers of patient-derived mesothelial cells than ES-2/mock transfectants. When these cells were inoculated into the abdominal cavity of female nude mice, mice that had received the transfectants showed better survival. When the transfectants were mixed with sialidase and injected, the survival was poorer, whereas when they were mixed with <i>N</i>-acetyl-2,3-dehydro-2-deoxyneuraminic acid, a sialidase inhibitor, the survival was significantly prolonged. These behaviors, concerned with peritoneal implantation and dissemination observed in vitro and in vivo, were dependent on the expression of MUC1. Therefore, sialic acid linked to MUC1 in the form, at least in part, of sialyl-T, as shown to be recognized by monoclonal antibody MY.1E12, is responsible for the suppression of adhesion of these cells to mesothelial cells and the suppression of peritoneal implantation and dissemination. |
| first_indexed | 2024-03-10T06:54:25Z |
| format | Article |
| id | doaj.art-c70e0b8395f348d0949d7a1cc3697f4f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:54:25Z |
| publishDate | 2021-10-01 |
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| series | Molecules |
| spelling | doaj.art-c70e0b8395f348d0949d7a1cc3697f4f2023-11-22T16:35:14ZengMDPI AGMolecules1420-30492021-10-012619596210.3390/molecules26195962A Possible Inhibitory Role of Sialic Acid on MUC1 in Peritoneal Dissemination of Clear Cell-Type Ovarian Cancer CellsYutaka Tamada0Hiroyuki Nomura1Daisuke Aoki2Tatsuro Irimura3Department of Gynecology, Jyoban Hospital, Tokiwa Foundation, Iwaki 972-8322, JapanDepartment of Obstetrics and Gynecology, Fujita Health University, Toyoake 470-1192, JapanDepartment of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo 160-8582, JapanDivision of Glycobiologics, Intractable Disease Research Center, Graduate School of Medicine, Juntendo University, Tokyo 113-8421, JapanThe role of sialic acids on MUC1 in peritoneal dissemination of ovarian cancer cells was investigated. A human ovarian carcinoma cell line, ES-2, was transfected with full-length MUC1 containing 22 or 42 tandem repeats. These transfectants were less adherent to monolayers of patient-derived mesothelial cells than ES-2/mock transfectants. When these cells were inoculated into the abdominal cavity of female nude mice, mice that had received the transfectants showed better survival. When the transfectants were mixed with sialidase and injected, the survival was poorer, whereas when they were mixed with <i>N</i>-acetyl-2,3-dehydro-2-deoxyneuraminic acid, a sialidase inhibitor, the survival was significantly prolonged. These behaviors, concerned with peritoneal implantation and dissemination observed in vitro and in vivo, were dependent on the expression of MUC1. Therefore, sialic acid linked to MUC1 in the form, at least in part, of sialyl-T, as shown to be recognized by monoclonal antibody MY.1E12, is responsible for the suppression of adhesion of these cells to mesothelial cells and the suppression of peritoneal implantation and dissemination.https://www.mdpi.com/1420-3049/26/19/5962mucinsneuraminidaseovarian neoplasmsperitoneal disseminationsialic acid |
| spellingShingle | Yutaka Tamada Hiroyuki Nomura Daisuke Aoki Tatsuro Irimura A Possible Inhibitory Role of Sialic Acid on MUC1 in Peritoneal Dissemination of Clear Cell-Type Ovarian Cancer Cells Molecules mucins neuraminidase ovarian neoplasms peritoneal dissemination sialic acid |
| title | A Possible Inhibitory Role of Sialic Acid on MUC1 in Peritoneal Dissemination of Clear Cell-Type Ovarian Cancer Cells |
| title_full | A Possible Inhibitory Role of Sialic Acid on MUC1 in Peritoneal Dissemination of Clear Cell-Type Ovarian Cancer Cells |
| title_fullStr | A Possible Inhibitory Role of Sialic Acid on MUC1 in Peritoneal Dissemination of Clear Cell-Type Ovarian Cancer Cells |
| title_full_unstemmed | A Possible Inhibitory Role of Sialic Acid on MUC1 in Peritoneal Dissemination of Clear Cell-Type Ovarian Cancer Cells |
| title_short | A Possible Inhibitory Role of Sialic Acid on MUC1 in Peritoneal Dissemination of Clear Cell-Type Ovarian Cancer Cells |
| title_sort | possible inhibitory role of sialic acid on muc1 in peritoneal dissemination of clear cell type ovarian cancer cells |
| topic | mucins neuraminidase ovarian neoplasms peritoneal dissemination sialic acid |
| url | https://www.mdpi.com/1420-3049/26/19/5962 |
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