Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool
ABSTRACT: During the poultry sperm cryopreservation process, an excess of reactive oxygen species is generated resulting in oxidative stress which harms the quality of avian spermatozoa. To counteract this effect, the addition of exogenous antioxidants, such as Pectoliv-80A (a by-product of olive oi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124002098 |
| _version_ | 1797249238341517312 |
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| author | Esther Díaz Ruiz Antonio González Ariza José Manuel León Jurado Ander Arando Arbulu Juan Fernández-Bolaños Guzmán Alejandra Bermúdez Oria Juan Vicente Delgado Bermejo Francisco Javier Navas González |
| author_facet | Esther Díaz Ruiz Antonio González Ariza José Manuel León Jurado Ander Arando Arbulu Juan Fernández-Bolaños Guzmán Alejandra Bermúdez Oria Juan Vicente Delgado Bermejo Francisco Javier Navas González |
| author_sort | Esther Díaz Ruiz |
| collection | DOAJ |
| description | ABSTRACT: During the poultry sperm cryopreservation process, an excess of reactive oxygen species is generated resulting in oxidative stress which harms the quality of avian spermatozoa. To counteract this effect, the addition of exogenous antioxidants, such as Pectoliv-80A (a by-product of olive oil), to the cryopreservation diluent is interesting. For this purpose, 16 roosters belonging to the Utrerana avian breed were used. Six semen pools (from the 6 different replicates) were divided into 4 aliquots corresponding to different concentrations of Pectoliv-80A that were tested (0, 300, 400, and 500 μg/mL), and the cryopreservation process was carried out. To evaluate post-thawing semen quality, different parameters such as motility, membrane functionality, reactive oxygen species production, lipid peroxidation, and acrosome integrity were studied. A discriminant canonical analysis was used to determine both the differences between the Pectoliv-80A concentration groups and the discriminant power of the aforementioned parameter used for semen evaluation. Total motility and membrane functionality were reported to be the most discriminant variables for differentiating the different antioxidant enrichment groups and concluded that concentrations of 300 μg/mL showed the most desirable quality of post-thawing semen. The present study could lead to the optimization of both cryopreservation and quality evaluation techniques of the sperm of rooster species, that support the conservation program of endangered local breeds. |
| first_indexed | 2024-04-24T20:27:18Z |
| format | Article |
| id | doaj.art-c72f2af3243544e4975387a2124f84b5 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-24T20:27:18Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-c72f2af3243544e4975387a2124f84b52024-03-22T05:38:25ZengElsevierPoultry Science0032-57912024-05-011035103630Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical toolEsther Díaz Ruiz0Antonio González Ariza1José Manuel León Jurado2Ander Arando Arbulu3Juan Fernández-Bolaños Guzmán4Alejandra Bermúdez Oria5Juan Vicente Delgado Bermejo6Francisco Javier Navas González7Department of Genetics, Faculty of Veterinary Sciences, University of Córdoba, Córdoba, 14071, SpainAgropecuary Provincial Centre, Diputación of Córdoba, Córdoba, 14071, Spain; Corresponding author:Agropecuary Provincial Centre, Diputación of Córdoba, Córdoba, 14071, SpainDepartment of Genetics, Faculty of Veterinary Sciences, University of Córdoba, Córdoba, 14071, SpainInstituto de la Grasa, Consejo Superior de Investigaciones Científicas, (CSIC), Sevilla, 41013, SpainInstituto de la Grasa, Consejo Superior de Investigaciones Científicas, (CSIC), Sevilla, 41013, SpainDepartment of Genetics, Faculty of Veterinary Sciences, University of Córdoba, Córdoba, 14071, SpainDepartment of Genetics, Faculty of Veterinary Sciences, University of Córdoba, Córdoba, 14071, SpainABSTRACT: During the poultry sperm cryopreservation process, an excess of reactive oxygen species is generated resulting in oxidative stress which harms the quality of avian spermatozoa. To counteract this effect, the addition of exogenous antioxidants, such as Pectoliv-80A (a by-product of olive oil), to the cryopreservation diluent is interesting. For this purpose, 16 roosters belonging to the Utrerana avian breed were used. Six semen pools (from the 6 different replicates) were divided into 4 aliquots corresponding to different concentrations of Pectoliv-80A that were tested (0, 300, 400, and 500 μg/mL), and the cryopreservation process was carried out. To evaluate post-thawing semen quality, different parameters such as motility, membrane functionality, reactive oxygen species production, lipid peroxidation, and acrosome integrity were studied. A discriminant canonical analysis was used to determine both the differences between the Pectoliv-80A concentration groups and the discriminant power of the aforementioned parameter used for semen evaluation. Total motility and membrane functionality were reported to be the most discriminant variables for differentiating the different antioxidant enrichment groups and concluded that concentrations of 300 μg/mL showed the most desirable quality of post-thawing semen. The present study could lead to the optimization of both cryopreservation and quality evaluation techniques of the sperm of rooster species, that support the conservation program of endangered local breeds.http://www.sciencedirect.com/science/article/pii/S0032579124002098genetic resourceantioxidantroostersemen quality assessmentdiscriminant canonical analysis |
| spellingShingle | Esther Díaz Ruiz Antonio González Ariza José Manuel León Jurado Ander Arando Arbulu Juan Fernández-Bolaños Guzmán Alejandra Bermúdez Oria Juan Vicente Delgado Bermejo Francisco Javier Navas González Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool Poultry Science genetic resource antioxidant rooster semen quality assessment discriminant canonical analysis |
| title | Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool |
| title_full | Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool |
| title_fullStr | Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool |
| title_full_unstemmed | Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool |
| title_short | Evaluation of the effect of the addition of an olive oil-derived antioxidant (Pectoliv-80A) in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool |
| title_sort | evaluation of the effect of the addition of an olive oil derived antioxidant pectoliv 80a in the extender for cryopreservation of rooster sperm through the use of a discriminant statistical tool |
| topic | genetic resource antioxidant rooster semen quality assessment discriminant canonical analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124002098 |
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