Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineering
Background: Tracheal reconstruction presents a challenge because of the difficulty in maintaining the rigidity of the trachea to ensure an open lumen and in achieving an intact luminal lining that secretes mucus to protect against infection.Methods: On the basis of the finding that tracheal cartilag...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1196521/full |
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| author | Ning Zeng Youbai Chen Yewen Wu Mengqing Zang Rene D. Largo Edward I. Chang Mark V. Schaverien Peirong Yu Qixu Zhang |
| author_facet | Ning Zeng Youbai Chen Yewen Wu Mengqing Zang Rene D. Largo Edward I. Chang Mark V. Schaverien Peirong Yu Qixu Zhang |
| author_sort | Ning Zeng |
| collection | DOAJ |
| description | Background: Tracheal reconstruction presents a challenge because of the difficulty in maintaining the rigidity of the trachea to ensure an open lumen and in achieving an intact luminal lining that secretes mucus to protect against infection.Methods: On the basis of the finding that tracheal cartilage has immune privilege, researchers recently started subjecting tracheal allografts to “partial decellularization” (in which only the epithelium and its antigenicity are removed), rather than complete decellularization, to maintain the tracheal cartilage as an ideal scaffold for tracheal tissue engineering and reconstruction. In the present study, we combined a bioengineering approach and a cryopreservation technique to fabricate a neo-trachea using pre-epithelialized cryopreserved tracheal allograft (ReCTA).Results: Our findings in rat heterotopic and orthotopic implantation models confirmed that tracheal cartilage has sufficient mechanical properties to bear neck movement and compression; indicated that pre-epithelialization with respiratory epithelial cells can prevent fibrosis obliteration and maintain lumen/airway patency; and showed that a pedicled adipose tissue flap can be easily integrated with a tracheal construct to achieve neovascularization.Conclusion: ReCTA can be pre-epithelialized and pre-vascularized using a 2-stage bioengineering approach and thus provides a promising strategy for tracheal tissue engineering. |
| first_indexed | 2024-04-09T14:17:48Z |
| format | Article |
| id | doaj.art-c746c28b4ddc424f91a3def5b683765c |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-09T14:17:48Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-c746c28b4ddc424f91a3def5b683765c2023-05-05T05:53:14ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-05-011110.3389/fbioe.2023.11965211196521Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineeringNing ZengYoubai ChenYewen WuMengqing ZangRene D. LargoEdward I. ChangMark V. SchaverienPeirong YuQixu ZhangBackground: Tracheal reconstruction presents a challenge because of the difficulty in maintaining the rigidity of the trachea to ensure an open lumen and in achieving an intact luminal lining that secretes mucus to protect against infection.Methods: On the basis of the finding that tracheal cartilage has immune privilege, researchers recently started subjecting tracheal allografts to “partial decellularization” (in which only the epithelium and its antigenicity are removed), rather than complete decellularization, to maintain the tracheal cartilage as an ideal scaffold for tracheal tissue engineering and reconstruction. In the present study, we combined a bioengineering approach and a cryopreservation technique to fabricate a neo-trachea using pre-epithelialized cryopreserved tracheal allograft (ReCTA).Results: Our findings in rat heterotopic and orthotopic implantation models confirmed that tracheal cartilage has sufficient mechanical properties to bear neck movement and compression; indicated that pre-epithelialization with respiratory epithelial cells can prevent fibrosis obliteration and maintain lumen/airway patency; and showed that a pedicled adipose tissue flap can be easily integrated with a tracheal construct to achieve neovascularization.Conclusion: ReCTA can be pre-epithelialized and pre-vascularized using a 2-stage bioengineering approach and thus provides a promising strategy for tracheal tissue engineering.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1196521/fulltracheal tissue engineeringtracheal cryopreservationpartial decellularizationpre-epithelializationvascularization |
| spellingShingle | Ning Zeng Youbai Chen Yewen Wu Mengqing Zang Rene D. Largo Edward I. Chang Mark V. Schaverien Peirong Yu Qixu Zhang Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineering Frontiers in Bioengineering and Biotechnology tracheal tissue engineering tracheal cryopreservation partial decellularization pre-epithelialization vascularization |
| title | Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineering |
| title_full | Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineering |
| title_fullStr | Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineering |
| title_full_unstemmed | Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineering |
| title_short | Pre-epithelialized cryopreserved tracheal allograft for neo-trachea flap engineering |
| title_sort | pre epithelialized cryopreserved tracheal allograft for neo trachea flap engineering |
| topic | tracheal tissue engineering tracheal cryopreservation partial decellularization pre-epithelialization vascularization |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1196521/full |
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