High-Efficiency Transformation and Expression of Genomic Libraries in Yeast

High-Efficiency Transformation and Expression of Genomic Libraries in Yeast

<i>Saccharomyces cerevisiae</i> is a powerful system for the expression of genome-wide or combinatorial libraries for diverse types of screening. However, expressing large libraries in yeast requires high-efficiency transformation and controlled expression. Transformation of yeast using...

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Bibliographic Details
Main Authors: Mira Loock, Luiza Berenguer Antunes, Rhiannon T Heslop, Antonio Alfonso De Lauri, Andressa Brito Lira, Igor Cestari
Format: Article
Language:English
Published: MDPI AG 2023-09-01
Series:Methods and Protocols
Subjects:
genomic library
electroporation
transformation
protein–ligand screening
proteome display
Online Access:https://www.mdpi.com/2409-9279/6/5/89

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https://www.mdpi.com/2409-9279/6/5/89

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