Considerations and practical implications of performing a phenotypic CRISPR/Cas survival screen.

Considerations and practical implications of performing a phenotypic CRISPR/Cas survival screen.

Genome-wide screens that have viability as a readout have been instrumental to identify essential genes. The development of gene knockout screens with the use of CRISPR-Cas has provided a more sensitive method to identify these genes. Here, we performed an exhaustive genome-wide CRISPR/Cas9 phenotyp...

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Bibliographic Details
Main Authors: Ator Ashoti, Francesco Limone, Melissa van Kranenburg, Anna Alemany, Mirna Baak, Judith Vivié, Frederica Piccioni, Pascale F Dijkers, Menno Creyghton, Kevin Eggan, Niels Geijsen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0263262

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https://doi.org/10.1371/journal.pone.0263262

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