Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model
ABSTRACT Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups: control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diab...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2021-08-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700200&tlng=en |
| _version_ | 1818490959835430912 |
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| author | Mirelly Marques Romeiro Santos Andressa Carolina Farias Pereira Subtil Cavalcante Luane Aparecida do Amaral Gabriel Henrique Oliveira de Souza Bárbara Suzuki dos Santos Luciane Candeloro Portugal Felipe Franscisco Bittencourt Junior Thiago Troquez Bruna Paola Murino Rafacho Priscila Aiko Hiane Elisvânia Freitas dos Santos |
| author_facet | Mirelly Marques Romeiro Santos Andressa Carolina Farias Pereira Subtil Cavalcante Luane Aparecida do Amaral Gabriel Henrique Oliveira de Souza Bárbara Suzuki dos Santos Luciane Candeloro Portugal Felipe Franscisco Bittencourt Junior Thiago Troquez Bruna Paola Murino Rafacho Priscila Aiko Hiane Elisvânia Freitas dos Santos |
| author_sort | Mirelly Marques Romeiro Santos |
| collection | DOAJ |
| description | ABSTRACT Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups: control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diabetic,n = 10); and STZ 40 mg/kg (diabetic, n = 10). DM2 was induced with a single intraperitoneal injection of STZ after four weeks of cafeteria diet in the three diabetic groups. All animals were evaluated as for anthropometric, and biochemical analyses, as well as liver, kidney and pancreas histological analyses. Results Lower weight gain, higher water intake, higher Lee index, hyperglycemia and modified total protein, urea, alpha-amylase, as well as insulin resistance, hepatic steatosis, pancreas, and kidney injury were observed in animals treated with 35 and 40 mg/kg of STZ. Conclusions The results show that the experimental model using cafeteria diet associated with 35 mg/kg of STZ is a low-cost model and efficient in order to develop DM2, confirmed by the presence of polydipsia, hyperglycemia, altered biochemical tests, insulin resistance and damages to the liver, pancreas and kidney, which is similar to the disease found in humans. |
| first_indexed | 2024-12-10T17:23:54Z |
| format | Article |
| id | doaj.art-c76a34dd1a4940359526467e271aacaa |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-10T17:23:54Z |
| publishDate | 2021-08-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-c76a34dd1a4940359526467e271aacaa2022-12-22T01:39:54ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502021-08-0136710.1590/acb360702Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes modelMirelly Marques Romeiro Santoshttps://orcid.org/0000-0002-1024-1540Andressa Carolina Farias Pereira Subtil Cavalcantehttps://orcid.org/0000-0003-4373-6707Luane Aparecida do Amaralhttps://orcid.org/0000-0002-1448-2472Gabriel Henrique Oliveira de Souzahttps://orcid.org/0000-0002-6378-2930Bárbara Suzuki dos Santoshttps://orcid.org/0000-0002-6601-4793Luciane Candeloro Portugalhttps://orcid.org/0000-0001-9619-0010Felipe Franscisco Bittencourt Juniorhttps://orcid.org/0000-0001-8801-9934Thiago Troquezhttps://orcid.org/0000-0003-1090-6116Bruna Paola Murino Rafachohttps://orcid.org/0000-0001-8825-6980Priscila Aiko Hianehttps://orcid.org/0000-0003-1115-4083Elisvânia Freitas dos Santoshttps://orcid.org/0000-0002-1528-6035ABSTRACT Purpose To develop a model of induction of type-2 diabetes (DM2) by combining low doses of streptozotocin (STZ) and a cafeteria diet. Methods Forty male Wistar rats (200 g) were allocated into four groups: control (non-diabetic, n = 10); STZ 30 mg/kg (diabetic, n = 10); STZ 35 mg/kg (diabetic,n = 10); and STZ 40 mg/kg (diabetic, n = 10). DM2 was induced with a single intraperitoneal injection of STZ after four weeks of cafeteria diet in the three diabetic groups. All animals were evaluated as for anthropometric, and biochemical analyses, as well as liver, kidney and pancreas histological analyses. Results Lower weight gain, higher water intake, higher Lee index, hyperglycemia and modified total protein, urea, alpha-amylase, as well as insulin resistance, hepatic steatosis, pancreas, and kidney injury were observed in animals treated with 35 and 40 mg/kg of STZ. Conclusions The results show that the experimental model using cafeteria diet associated with 35 mg/kg of STZ is a low-cost model and efficient in order to develop DM2, confirmed by the presence of polydipsia, hyperglycemia, altered biochemical tests, insulin resistance and damages to the liver, pancreas and kidney, which is similar to the disease found in humans.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700200&tlng=enLiverHyperglycemiaBody WeightPancreasModelsAnimal |
| spellingShingle | Mirelly Marques Romeiro Santos Andressa Carolina Farias Pereira Subtil Cavalcante Luane Aparecida do Amaral Gabriel Henrique Oliveira de Souza Bárbara Suzuki dos Santos Luciane Candeloro Portugal Felipe Franscisco Bittencourt Junior Thiago Troquez Bruna Paola Murino Rafacho Priscila Aiko Hiane Elisvânia Freitas dos Santos Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model Acta Cirúrgica Brasileira Liver Hyperglycemia Body Weight Pancreas Models Animal |
| title | Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
| title_full | Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
| title_fullStr | Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
| title_full_unstemmed | Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
| title_short | Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model |
| title_sort | combination of cafeteria diet with intraperitoneally streptozotocin in rats a type 2 diabetes model |
| topic | Liver Hyperglycemia Body Weight Pancreas Models Animal |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021000700200&tlng=en |
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