Executing cell-specific cross-linking immunoprecipitation and sequencing (seCLIP) in C. elegans

Summary: The single-end enhanced cross-linking immunoprecipitation (seCLIP) method is well suited for efficient and unbiased transcriptome-wide interrogation of RNA-binding protein (RBP) interaction sites. Here, we provide a protocol for executing cell-specific seCLIP for any desired RBP in Caenorha...

Full description

Bibliographic Details
Main Authors: Stephen M. Blazie, Yishi Jin
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008395