Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo
Long acquisition time and high illumination intensities needed in super-resolution imaging often generate photobleaching and phototoxicity. Here the authors develop a non-deterministic scanning approach based on a real-time feedback system that enables faster acquisition with lower light doses for i...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2019-02-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-019-08442-4 |