Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo

Long acquisition time and high illumination intensities needed in super-resolution imaging often generate photobleaching and phototoxicity. Here the authors develop a non-deterministic scanning approach based on a real-time feedback system that enables faster acquisition with lower light doses for i...

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Bibliographic Details
Main Authors: Jes Dreier, Marco Castello, Giovanna Coceano, Rodrigo Cáceres, Julie Plastino, Giuseppe Vicidomini, Ilaria Testa
Format: Article
Language:English
Published: Nature Portfolio 2019-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-08442-4