DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium Complexes
With the development of metal-based drugs, Ru(II) compounds present potential applications of PDT (photodynamic therapy) and anticancer reagents. We herein synthesized two naphthyl-appended ruthenium complexes by the combination of the ligand with naphthyl and bipyridyl. The DNA affinities, photocle...
| Main Authors: | , , , , |
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| פורמט: | Article |
| שפה: | English |
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MDPI AG
2022-06-01
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| סדרה: | Molecules |
| נושאים: | |
| גישה מקוונת: | https://www.mdpi.com/1420-3049/27/12/3676 |
| _version_ | 1827658200094081024 |
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| author | Xia Hu Qian Luo Yao Qin Yao Wu Xue-Wen Liu |
| author_facet | Xia Hu Qian Luo Yao Qin Yao Wu Xue-Wen Liu |
| author_sort | Xia Hu |
| collection | DOAJ |
| description | With the development of metal-based drugs, Ru(II) compounds present potential applications of PDT (photodynamic therapy) and anticancer reagents. We herein synthesized two naphthyl-appended ruthenium complexes by the combination of the ligand with naphthyl and bipyridyl. The DNA affinities, photocleavage abilities, and photocytotoxicity were studied by various spectral methods, viscosity measurement, theoretical computation method, gel electrophoresis, and MTT method. Two complexes exhibited strong interaction with calf thymus DNA by intercalation. Production of singlet oxygen (<sup>1</sup>O<sub>2</sub>) led to obvious DNA photocleavage activities of two complexes under 365 nm light. Furthermore, two complexes displayed obvious photocytotoxicity and low dark cytotoxicity towards Hela, A549, and A375 cells. |
| first_indexed | 2024-03-09T22:57:28Z |
| format | Article |
| id | doaj.art-c79c7bcd159b4df08885a8ab1e6e0111 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T22:57:28Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c79c7bcd159b4df08885a8ab1e6e01112023-11-23T18:09:28ZengMDPI AGMolecules1420-30492022-06-012712367610.3390/molecules27123676DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium ComplexesXia Hu0Qian Luo1Yao Qin2Yao Wu3Xue-Wen Liu4Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, ChinaHunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, ChinaHunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, ChinaHunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, ChinaHunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, ChinaWith the development of metal-based drugs, Ru(II) compounds present potential applications of PDT (photodynamic therapy) and anticancer reagents. We herein synthesized two naphthyl-appended ruthenium complexes by the combination of the ligand with naphthyl and bipyridyl. The DNA affinities, photocleavage abilities, and photocytotoxicity were studied by various spectral methods, viscosity measurement, theoretical computation method, gel electrophoresis, and MTT method. Two complexes exhibited strong interaction with calf thymus DNA by intercalation. Production of singlet oxygen (<sup>1</sup>O<sub>2</sub>) led to obvious DNA photocleavage activities of two complexes under 365 nm light. Furthermore, two complexes displayed obvious photocytotoxicity and low dark cytotoxicity towards Hela, A549, and A375 cells.https://www.mdpi.com/1420-3049/27/12/3676ruthenium complexDNA interactionphotocleavagemolecular dockingphotocytotoxicity |
| spellingShingle | Xia Hu Qian Luo Yao Qin Yao Wu Xue-Wen Liu DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium Complexes Molecules ruthenium complex DNA interaction photocleavage molecular docking photocytotoxicity |
| title | DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium Complexes |
| title_full | DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium Complexes |
| title_fullStr | DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium Complexes |
| title_full_unstemmed | DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium Complexes |
| title_short | DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium Complexes |
| title_sort | dna interaction dna photocleavage photocytotoxicity in vitro and molecular docking of naphthyl appended ruthenium complexes |
| topic | ruthenium complex DNA interaction photocleavage molecular docking photocytotoxicity |
| url | https://www.mdpi.com/1420-3049/27/12/3676 |
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