Benchmarking laboratory processes to characterise low-biomass respiratory microbiota

Abstract The low biomass of respiratory samples makes it difficult to accurately characterise the microbial community composition. PCR conditions and contaminating microbial DNA can alter the biological profile. The objective of this study was to benchmark the currently available laboratory protocol...

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Bibliographic Details
Main Authors: Raiza Hasrat, Jolanda Kool, Wouter A. A. de Steenhuijsen Piters, Mei Ling J. N. Chu, Sjoerd Kuiling, James A. Groot, Elske M. van Logchem, Susana Fuentes, Eelco Franz, Debby Bogaert, Thijs Bosch
Format: Article
Language:English
Published: Nature Portfolio 2021-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-96556-5