Benchmarking laboratory processes to characterise low-biomass respiratory microbiota
Abstract The low biomass of respiratory samples makes it difficult to accurately characterise the microbial community composition. PCR conditions and contaminating microbial DNA can alter the biological profile. The objective of this study was to benchmark the currently available laboratory protocol...
Main Authors: | Raiza Hasrat, Jolanda Kool, Wouter A. A. de Steenhuijsen Piters, Mei Ling J. N. Chu, Sjoerd Kuiling, James A. Groot, Elske M. van Logchem, Susana Fuentes, Eelco Franz, Debby Bogaert, Thijs Bosch |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2021-08-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-96556-5 |
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