Constant Pressure-Regulated Microdroplet Polymerase Chain Reaction in Microfluid Chips: A Methodological Study

Digital polymerase chain reaction (PCR) technology in microfluidic systems often results in bubble formation post-amplification, leading to microdroplet fragmentation and compromised detection accuracy. To solve this issue, this study introduces a method based on the constant pressure regulation of...

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Bibliographic Details
Main Authors: Luyang Duanmu, Youji Shen, Ping Gong, Hao Zhang, Xiangkai Meng, Yuanhua Yu
Format: Article
Language:English
Published: MDPI AG 2023-12-01
Series:Micromachines
Subjects:
Online Access:https://www.mdpi.com/2072-666X/15/1/8