Celastrol and Melatonin Modify <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative Stress
An excess of oxidative stress (OS) may affect several physiological processes fundamental to reproduction. SIRT1, SIRT6 and SIRT7 are involved in protection stress systems caused by OS, and they can be activated by antioxidants such as celastrol or melatonin. In this study, we evaluate <i>SIRT...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/12/1871 |
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| author | Rita Martín-Ramírez Rebeca González-Fernández Jairo Hernández Pablo Martín-Vasallo Angela Palumbo Julio Ávila |
| author_facet | Rita Martín-Ramírez Rebeca González-Fernández Jairo Hernández Pablo Martín-Vasallo Angela Palumbo Julio Ávila |
| author_sort | Rita Martín-Ramírez |
| collection | DOAJ |
| description | An excess of oxidative stress (OS) may affect several physiological processes fundamental to reproduction. SIRT1, SIRT6 and SIRT7 are involved in protection stress systems caused by OS, and they can be activated by antioxidants such as celastrol or melatonin. In this study, we evaluate <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> gene expression in cultured human granulosa-lutein (hGL) cells in response to OS inductors (glucose or peroxynitrite) and/or antioxidants. Our results show that celastrol and melatonin improve cell survival in the presence and absence of OS inductors. In addition, melatonin induced <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> gene expression while celastrol only induced <i>SIRT7</i> gene expression. This response was not altered by the addition of OS inductors. Our previous data for cultured hGL cells showed a dual role of celastrol as a free radical scavenger and as a protective agent by regulating gene expression. This study shows a direct effect of celastrol on <i>SIRT7</i> gene expression. Melatonin may protect from OS in a receptor-mediated manner rather than as a scavenger. In conclusion, our results show increased hGL cells survival with melatonin or celastrol treatment under OS conditions, probably through the regulation of nuclear sirtuins’ gene expression. |
| first_indexed | 2024-03-10T04:38:56Z |
| format | Article |
| id | doaj.art-c7d6ec1c835d4e0f902bd13a82d4a537 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T04:38:56Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-c7d6ec1c835d4e0f902bd13a82d4a5372023-11-23T03:32:13ZengMDPI AGAntioxidants2076-39212021-11-011012187110.3390/antiox10121871Celastrol and Melatonin Modify <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative StressRita Martín-Ramírez0Rebeca González-Fernández1Jairo Hernández2Pablo Martín-Vasallo3Angela Palumbo4Julio Ávila5Laboratorio de Biología del Desarrollo, UDI de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, 38206 La Laguna, SpainLaboratorio de Biología del Desarrollo, UDI de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, 38206 La Laguna, SpainCentro de Asistencia a la Reproducción Humana de Canarias, 38202 La Laguna, SpainLaboratorio de Biología del Desarrollo, UDI de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, 38206 La Laguna, SpainCentro de Asistencia a la Reproducción Humana de Canarias, 38202 La Laguna, SpainLaboratorio de Biología del Desarrollo, UDI de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, 38206 La Laguna, SpainAn excess of oxidative stress (OS) may affect several physiological processes fundamental to reproduction. SIRT1, SIRT6 and SIRT7 are involved in protection stress systems caused by OS, and they can be activated by antioxidants such as celastrol or melatonin. In this study, we evaluate <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> gene expression in cultured human granulosa-lutein (hGL) cells in response to OS inductors (glucose or peroxynitrite) and/or antioxidants. Our results show that celastrol and melatonin improve cell survival in the presence and absence of OS inductors. In addition, melatonin induced <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> gene expression while celastrol only induced <i>SIRT7</i> gene expression. This response was not altered by the addition of OS inductors. Our previous data for cultured hGL cells showed a dual role of celastrol as a free radical scavenger and as a protective agent by regulating gene expression. This study shows a direct effect of celastrol on <i>SIRT7</i> gene expression. Melatonin may protect from OS in a receptor-mediated manner rather than as a scavenger. In conclusion, our results show increased hGL cells survival with melatonin or celastrol treatment under OS conditions, probably through the regulation of nuclear sirtuins’ gene expression.https://www.mdpi.com/2076-3921/10/12/1871melatonincelastrolsirtuinsoxidative stressgranulosa-lutein cells |
| spellingShingle | Rita Martín-Ramírez Rebeca González-Fernández Jairo Hernández Pablo Martín-Vasallo Angela Palumbo Julio Ávila Celastrol and Melatonin Modify <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative Stress Antioxidants melatonin celastrol sirtuins oxidative stress granulosa-lutein cells |
| title | Celastrol and Melatonin Modify <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative Stress |
| title_full | Celastrol and Melatonin Modify <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative Stress |
| title_fullStr | Celastrol and Melatonin Modify <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative Stress |
| title_full_unstemmed | Celastrol and Melatonin Modify <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative Stress |
| title_short | Celastrol and Melatonin Modify <i>SIRT1</i>, <i>SIRT6</i> and <i>SIRT7</i> Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative Stress |
| title_sort | celastrol and melatonin modify i sirt1 i i sirt6 i and i sirt7 i gene expression and improve the response of human granulosa lutein cells to oxidative stress |
| topic | melatonin celastrol sirtuins oxidative stress granulosa-lutein cells |
| url | https://www.mdpi.com/2076-3921/10/12/1871 |
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